This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2ccq
From Proteopedia
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
| - | The AAA ATPase p97 is a ubiquitin-selective molecular machine involved in, multiple cellular processes, including protein degradation through the, ubiquitin-proteasome system and homotypic membrane fusion. Specific p97, functions are mediated by a variety of cofactors, among them peptide, N-glycanase, an enzyme that removes glycans from misfolded glycoproteins., Here we report the three-dimensional structure of the aminoterminal PUB, domain of human peptide N-glycanase. We demonstrate that the PUB domain is, a novel p97 binding module interacting with the D1 and/or D2 ATPase, domains of p97 and identify an evolutionary conserved surface patch, required for p97 binding. Furthermore, we show that the PUB and UBX, domains do not bind to p97 in a mutually exclusive manner. Our results, . | + | The AAA ATPase p97 is a ubiquitin-selective molecular machine involved in, multiple cellular processes, including protein degradation through the, ubiquitin-proteasome system and homotypic membrane fusion. Specific p97, functions are mediated by a variety of cofactors, among them peptide, N-glycanase, an enzyme that removes glycans from misfolded glycoproteins., Here we report the three-dimensional structure of the aminoterminal PUB, domain of human peptide N-glycanase. We demonstrate that the PUB domain is, a novel p97 binding module interacting with the D1 and/or D2 ATPase, domains of p97 and identify an evolutionary conserved surface patch, required for p97 binding. Furthermore, we show that the PUB and UBX, domains do not bind to p97 in a mutually exclusive manner. Our results, suggest that PUB domain-containing proteins constitute a widespread family, of diverse p97 cofactors. |
==About this Structure== | ==About this Structure== | ||
| - | 2CCQ is a | + | 2CCQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CCQ OCA]. |
==Reference== | ==Reference== | ||
| Line 21: | Line 21: | ||
[[Category: glycoprotein]] | [[Category: glycoprotein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:48:33 2007'' |
Revision as of 10:43, 5 November 2007
|
THE PUB DOMAIN FUNCTIONS AS A P97 BINDING MODULE IN HUMAN PEPTIDE N-GLYCANASE.
Overview
The AAA ATPase p97 is a ubiquitin-selective molecular machine involved in, multiple cellular processes, including protein degradation through the, ubiquitin-proteasome system and homotypic membrane fusion. Specific p97, functions are mediated by a variety of cofactors, among them peptide, N-glycanase, an enzyme that removes glycans from misfolded glycoproteins., Here we report the three-dimensional structure of the aminoterminal PUB, domain of human peptide N-glycanase. We demonstrate that the PUB domain is, a novel p97 binding module interacting with the D1 and/or D2 ATPase, domains of p97 and identify an evolutionary conserved surface patch, required for p97 binding. Furthermore, we show that the PUB and UBX, domains do not bind to p97 in a mutually exclusive manner. Our results, suggest that PUB domain-containing proteins constitute a widespread family, of diverse p97 cofactors.
About this Structure
2CCQ is a Single protein structure of sequence from Homo sapiens with GOL as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
The PUB domain functions as a p97 binding module in human peptide N-glycanase., Allen MD, Buchberger A, Bycroft M, J Biol Chem. 2006 Sep 1;281(35):25502-8. Epub 2006 Jun 28. PMID:16807242
Page seeded by OCA on Mon Nov 5 12:48:33 2007
