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1cbf
From Proteopedia
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|PDB= 1cbf |SIZE=350|CAPTION= <scene name='initialview01'>1cbf</scene>, resolution 2.4Å | |PDB= 1cbf |SIZE=350|CAPTION= <scene name='initialview01'>1cbf</scene>, resolution 2.4Å | ||
|SITE= <scene name='pdbsite=PAL:Phosphate+Ion+Involved+In+Crystal+Contact'>PAL</scene> and <scene name='pdbsite=SAH:S-Adenosyl-Homocysteine+Binding+Site.+Crystals+Formed+In+...'>SAH</scene> | |SITE= <scene name='pdbsite=PAL:Phosphate+Ion+Involved+In+Crystal+Contact'>PAL</scene> and <scene name='pdbsite=SAH:S-Adenosyl-Homocysteine+Binding+Site.+Crystals+Formed+In+...'>SAH</scene> | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> | + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Precorrin-4_C(11)-methyltransferase Precorrin-4 C(11)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.133 2.1.1.133] </span> |
|GENE= CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium]) | |GENE= CBIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1404 Bacillus megaterium]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbf OCA], [http://www.ebi.ac.uk/pdbsum/1cbf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cbf RCSB]</span> | ||
}} | }} | ||
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[[Category: Wilson, K S.]] | [[Category: Wilson, K S.]] | ||
[[Category: Woodcock, S C.]] | [[Category: Woodcock, S C.]] | ||
| - | [[Category: PO4]] | ||
| - | [[Category: SAH]] | ||
[[Category: cobalamin biosynthesis]] | [[Category: cobalamin biosynthesis]] | ||
[[Category: methylase]] | [[Category: methylase]] | ||
[[Category: precorrin-4 methyltransferase]] | [[Category: precorrin-4 methyltransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:18:21 2008'' |
Revision as of 16:18, 30 March 2008
| |||||||
| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | , | ||||||
| Gene: | CBIF (Bacillus megaterium) | ||||||
| Activity: | Precorrin-4 C(11)-methyltransferase, with EC number 2.1.1.133 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE X-RAY STRUCTURE OF A COBALAMIN BIOSYNTHETIC ENZYME, COBALT PRECORRIN-4 METHYLTRANSFERASE, CBIF
Overview
Biosynthesis of the corrin ring of vitamin B12 requires the action of six S-adenosyl-L-methionine (AdoMet) dependent transmethylases, closely related in sequence. The first X-ray structure of one of these, cobalt-precorrin-4 transmethylase, CbiF, from Bacillus megaterium has been determined to a resolution of 2.4 A. CbiF contains two alphabeta domains forming a trough in which S-adenosyl-L-homocysteine (AdoHcy) binds. The location of AdoHcy and a number of conserved residues, helps define the precorrin binding site. A second crystal form determined at 3.1 A resolution highlights the flexibility of two loops around this site. CbiF employs a unique mode of AdoHcy binding and represents a new class of transmethylase.
About this Structure
1CBF is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.
Reference
The X-ray structure of a cobalamin biosynthetic enzyme, cobalt-precorrin-4 methyltransferase., Schubert HL, Wilson KS, Raux E, Woodcock SC, Warren MJ, Nat Struct Biol. 1998 Jul;5(7):585-92. PMID:9665173
Page seeded by OCA on Sun Mar 30 19:18:21 2008
