2cfp
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 2CFP is a | + | 2CFP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC2. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transport mechanism]] | [[Category: transport mechanism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:38:51 2007'' |
Revision as of 10:33, 5 November 2007
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SUGAR FREE LACTOSE PERMEASE AT ACIDIC PH
Overview
Cation-coupled active transport is an essential cellular process found, ubiquitously in all living organisms. Here, we present two novel, ligand-free X-ray structures of the lactose permease (LacY) of Escherichia, coli determined at acidic and neutral pH, and propose a model for the, mechanism of coupling between lactose and H+ translocation. No, sugar-binding site is observed in the absence of ligand, and deprotonation, of the key residue Glu269 is associated with ligand binding. Thus, substrate induces formation of the sugar-binding site, as well as the, initial step in H+ transduction.
About this Structure
2CFP is a Single protein structure of sequence from Escherichia coli with HG as ligand. Structure known Active Site: AC2. Full crystallographic information is available from OCA.
Reference
Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY., Mirza O, Guan L, Verner G, Iwata S, Kaback HR, EMBO J. 2006 Mar 22;25(6):1177-83. Epub 2006 Mar 9. PMID:16525509
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