1cld
From Proteopedia
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|PDB= 1cld |SIZE=350|CAPTION= <scene name='initialview01'>1cld</scene> | |PDB= 1cld |SIZE=350|CAPTION= <scene name='initialview01'>1cld</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CD:CADMIUM ION'>CD</scene> | + | |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cld OCA], [http://www.ebi.ac.uk/pdbsum/1cld PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cld RCSB]</span> | ||
}} | }} | ||
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[[Category: Coleman, J E.]] | [[Category: Coleman, J E.]] | ||
[[Category: Gardner, K H.]] | [[Category: Gardner, K H.]] | ||
| - | [[Category: CD]] | ||
[[Category: zinc-binding domain]] | [[Category: zinc-binding domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:23:55 2008'' |
Revision as of 16:23, 30 March 2008
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA-BINDING PROTEIN
Overview
The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.
About this Structure
1CLD is a Single protein structure of sequence from Kluyveromyces lactis. Full crystallographic information is available from OCA.
Reference
Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain., Gardner KH, Anderson SF, Coleman JE, Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715
Page seeded by OCA on Sun Mar 30 19:23:55 2008
