4ur2
From Proteopedia
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| - | ''' | + | ==Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with iodide== |
| + | <StructureSection load='4ur2' size='340' side='right' caption='[[4ur2]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4ur2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfurospirillum_multivorans Sulfurospirillum multivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UR2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UR2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BVQ:NORPSEUDO-B12'>BVQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uqu|4uqu]], [[4ur0|4ur0]], [[4ur1|4ur1]], [[4ur3|4ur3]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tetrachloroethene_reductive_dehalogenase Tetrachloroethene reductive dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.8 1.97.1.8] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ur2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ur2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ur2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ur2 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region. | ||
| - | + | Structural basis for organohalide respiration.,Bommer M, Kunze C, Fesseler J, Schubert T, Diekert G, Dobbek H Science. 2014 Oct 2. pii: 1258118. PMID:25278505<ref>PMID:25278505</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Sulfurospirillum multivorans]] | ||
| + | [[Category: Tetrachloroethene reductive dehalogenase]] | ||
| + | [[Category: Bommer, M.]] | ||
| + | [[Category: Diekert, G.]] | ||
| + | [[Category: Dobbek, H.]] | ||
| + | [[Category: Fesseler, J.]] | ||
| + | [[Category: Kunze, C.]] | ||
| + | [[Category: Schubert, T.]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 10:45, 20 October 2014
Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with iodide
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