1cxl
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1cxl |SIZE=350|CAPTION= <scene name='initialview01'>1cxl</scene>, resolution 1.810Å | |PDB= 1cxl |SIZE=350|CAPTION= <scene name='initialview01'>1cxl</scene>, resolution 1.810Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=G4D:4-DEOXY-ALPHA-D-GLUCOSE'>G4D</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=GLF:1-FLUORO-ALPHA-1-DEOXY-D-GLUCOSE'>GLF</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxl OCA], [http://www.ebi.ac.uk/pdbsum/1cxl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cxl RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Dijkstra, B W.]] | [[Category: Dijkstra, B W.]] | ||
[[Category: Uitdehaag, J C.M.]] | [[Category: Uitdehaag, J C.M.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: MPD]] | ||
[[Category: 4- deoxymaltotriose]] | [[Category: 4- deoxymaltotriose]] | ||
[[Category: alpha-amylase family]] | [[Category: alpha-amylase family]] | ||
| Line 32: | Line 33: | ||
[[Category: intermediate complex]] | [[Category: intermediate complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:34 2008'' |
Revision as of 16:30, 30 March 2008
| |||||||
| , resolution 1.810Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Activity: | Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMPLEX BETWEEN A COVALENT INTERMEDIATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q
Overview
Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the alpha-amylase family, which uses a double displacement mechanism to process alpha-linked glucose polymers. We have determined two X-ray structures of CGTase complexes, one with an intact substrate at 2.1 A resolution, and the other with a covalently bound reaction intermediate at 1.8 A resolution. These structures give evidence for substrate distortion and the covalent character of the intermediate and for the first time show, in atomic detail, how catalysis in the alpha-amylase family proceeds by the concerted action of all active site residues.
About this Structure
1CXL is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.
Reference
X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family., Uitdehaag JC, Mosi R, Kalk KH, van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW, Nat Struct Biol. 1999 May;6(5):432-6. PMID:10331869
Page seeded by OCA on Sun Mar 30 19:30:34 2008
