1cz0
From Proteopedia
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|PDB= 1cz0 |SIZE=350|CAPTION= <scene name='initialview01'>1cz0</scene>, resolution 2.1Å | |PDB= 1cz0 |SIZE=350|CAPTION= <scene name='initialview01'>1cz0</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1cyq|1CYQ]], [[1a73|1A73]], [[1a74|1A74]], [[1ipp|1IPP]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cz0 OCA], [http://www.ebi.ac.uk/pdbsum/1cz0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cz0 RCSB]</span> | ||
}} | }} | ||
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[[Category: Jurica, M S.]] | [[Category: Jurica, M S.]] | ||
[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: ZN]] | ||
[[Category: beta sheet dna binding]] | [[Category: beta sheet dna binding]] | ||
[[Category: distorted dna]] | [[Category: distorted dna]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:31:09 2008'' |
Revision as of 16:31, 30 March 2008
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| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Related: | 1CYQ, 1A73, 1A74, 1IPP
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION
Overview
A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction.
About this Structure
1CZ0 is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
A novel endonuclease mechanism directly visualized for I-PpoI., Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:10581547
Page seeded by OCA on Sun Mar 30 19:31:09 2008
