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| - | ==Structure of the ribosome-SecYE complex in the membrane environment==
| + | #REDIRECT [[4v6m]] This PDB entry is obsolete and replaced by 4v6m |
| - | <StructureSection load='3j01' size='340' side='right' caption='[[3j01]], [[Resolution|resolution]] 7.10Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[3j01]] is a 33 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecol5 Ecol5], [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Escherichia_coli_dh1 Escherichia coli dh1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J01 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J01 FirstGlance]. <br>
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| - | </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j00|3j00]]</td></tr>
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| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j01 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j01 RCSB], [http://www.ebi.ac.uk/pdbsum/3j01 PDBsum]</span></td></tr>
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| - | <table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The ubiquitous SecY-Sec61 complex translocates nascent secretory proteins across cellular membranes and integrates membrane proteins into lipid bilayers. Several structures of mostly detergent-solubilized Sec complexes have been reported. Here we present a single-particle cryo-EM structure of the SecYEG complex in a membrane environment, bound to a translating ribosome, at subnanometer resolution. Using the SecYEG complex reconstituted in a so-called Nanodisc, we could trace the nascent polypeptide chain from the peptidyltransferase center into the membrane. The reconstruction allowed for the identification of ribosome-lipid interactions. The rRNA helix 59 (H59) directly contacts the lipid surface and appears to modulate the membrane in immediate vicinity to the proposed lateral gate of the protein-conducting channel (PCC). On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane.
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| - | Cryo-EM structure of the ribosome-SecYE complex in the membrane environment.,Frauenfeld J, Gumbart J, Sluis EO, Funes S, Gartmann M, Beatrix B, Mielke T, Berninghausen O, Becker T, Schulten K, Beckmann R Nat Struct Mol Biol. 2011 May;18(5):614-21. Epub 2011 Apr 17. PMID:21499241<ref>PMID:21499241</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | ==See Also==
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| - | *[[Preprotein translocase|Preprotein translocase]]
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| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Ecol5]]
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| - | [[Category: Ecoli]]
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| - | [[Category: Escherichia coli dh1]]
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| - | [[Category: Beatrix, B.]]
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| - | [[Category: Becker, T.]]
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| - | [[Category: Beckmann, R.]]
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| - | [[Category: Berninghausen, O.]]
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| - | [[Category: Frauenfeld, J.]]
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| - | [[Category: Funes, S.]]
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| - | [[Category: Gartmann, M.]]
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| - | [[Category: Gumbart, J.]]
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| - | [[Category: Mielke, T.]]
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| - | [[Category: Schulten, K.]]
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| - | [[Category: Sluis, E O.van der.]]
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| - | [[Category: 70s ribosome]]
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| - | [[Category: Nanodisc]]
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| - | [[Category: Nucleotide-binding]]
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| - | [[Category: Protein biosynthesis]]
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| - | [[Category: Ribonucleoprotein]]
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| - | [[Category: Ribosomal protein]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Ribosome-ribosomal protein complex]]
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| - | [[Category: Secyeg]]
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| - | [[Category: Translation]]
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| - | [[Category: Translocon]]
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| - | [[Category: Zinc-finger]]
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