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| - | ==Crystal structure of the E. coli ribosome bound to linopristin. This file contains the 50S subunit of the second 70S ribosome with linopristin bound.==
| + | #REDIRECT [[4u1v]] This PDB entry is obsolete and replaced by 4u1v |
| - | <StructureSection load='4too' size='340' side='right' caption='[[4too]], [[Resolution|resolution]] 3.00Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[4too]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Escherichia_coli_str._k-12_substr._mds42 Escherichia coli str. k-12 substr. mds42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TOO FirstGlance]. <br>
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| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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| - | <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=004:(2S)-AMINO(PHENYL)ETHANOIC+ACID'>004</scene>, <scene name='pdbligand=04X:(2S)-5-(MORPHOLIN-4-YLMETHYL)-1,2,3,6-TETRAHYDROPYRIDINE-2-CARBOXYLIC+ACID'>04X</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=MHU:4-N,N-(DIMETHYLAMINO)-L-PHENYLALANINE'>MHU</scene>, <scene name='pdbligand=MHW:3-HYDROXYPICOLINIC+ACID'>MHW</scene></td></tr>
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| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tol|4tol]], [[4tom|4tom]], [[4ton|4ton]]</td></tr>
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| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4too FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4too OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4too RCSB], [http://www.ebi.ac.uk/pdbsum/4too PDBsum]</span></td></tr>
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| - | <table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Streptogramin antibiotics are divided into type A and B streptogramins, which in combination can act synergistically. We compared the molecular interactions of the streptogramin combinations Synercid (type A: dalfopristin, type B: quinupristin) and NXL 103 (type A: flopristin, type B: linopristin) with the Escherichia coli 70S ribosome by x-ray crystallography. We further analyzed the activity of the streptogramin components individually and in combination. Streptogramin A and B components in Synercid and NXL 103 exhibit synergistic antimicrobial activity against certain pathogenic bacteria. However, in transcription-coupled translation assays, only combinations that include dalfopristin, the streptogramin A component of Synercid, show synergy. Notably, the diethylaminoethylsulfonyl group in dalfopristin reduces its activity, but is the basis for synergy in transcription-coupled translation assays before its rapid hydrolysis from the depsipeptide core. Replacement of the diethylaminoethylsulfonyl group in dalfopristin by a non-hydrolyzable group may therefore be beneficial for synergy. The absence of general streptogramin synergy in transcription-coupled translation assays suggests that synergistic antimicrobial activity of streptogramins can occur independently of streptogramin effects on translation.
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| - | Synergy of streptogramin antibiotics occurs independently of their effects on translation.,Noeske J, Huang J, Olivier NB, Giacobbe RA, Zambrowski M, Cate JH Antimicrob Agents Chemother. 2014 Jun 23. pii: AAC.03389-14. PMID:24957822<ref>PMID:24957822</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Escherichia coli str. k-12 substr. mds42]]
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| - | [[Category: Cate, J H.D.]]
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| - | [[Category: Giacobbe, R A.]]
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| - | [[Category: Huang, J.]]
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| - | [[Category: Noeske, J.]]
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| - | [[Category: Olivier, N B.]]
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| - | [[Category: Zambrowski, M.]]
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| - | [[Category: 23]]
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| - | [[Category: 50]]
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| - | [[Category: 70]]
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| - | [[Category: Exit]]
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| - | [[Category: Peptidyl]]
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| - | [[Category: Protein biosynthesis]]
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| - | [[Category: Ribosomal subunit]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Rna]]
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| - | [[Category: Transfer]]
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| - | [[Category: Trna]]
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