Nitrile hydratase

Function

Nitrile hydratase (NH) catalyze the hydration of nitriles to amides. NH contains Fe or Co in its active site. NH consists of two subunits: α and β and exists as dimer or tetramer with one metal ion bound per dimer.

Disease

Relevance

Nitrile hydratase is used industrially in the production of acrylamide and nicotinamide.

Structural highlights

3D structures of nitrile hydratase

Updated on 13-August-2014

1ahj, 2cyz, 2cz0, 2cz1 – ReNH α,β subunits + Fe – Rhodococcus erythropolis

2czf, 3a8l – ReNH α (mutant),β subunits + Fe

Nitrile hydratase complex

2ahj – ReNH α,β subunits + Fe + NO2
2cz6, 2d0q – ReNH α,β subunits + Fe + cyclohexyl isocyanate
2cz7, 2zpb – ReNH α,β subunits + Fe + NO
2qdy – ReNH α,β subunits + Fe + isobutyronitrile
2zpe, 2zpg, 2zph, 2zpi – ReNH α,β subunits + Fe + tert-butyl isocyanate
2zpf – ReNH α,β subunits + Fe + tert-butyl isocyanate + NO
3a8o – ReNH α,β subunits + Fe + trimethyl acetonitrile
3a8g – ReNH α (mutant),β subunits + Fe + trimethyl acetonitrile + NO
3a8h – ReNH α (mutant),β subunits + Fe + trimethyl acetonitrile
3a8m – ReNH α,β (mutant) subunits + Fe + trimethyl acetonitrile

References