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1vy8
From Proteopedia
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vy8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vy8 RCSB], [http://www.ebi.ac.uk/pdbsum/1vy8 PDBsum]</span></td></tr> | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vy8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vy8 RCSB], [http://www.ebi.ac.uk/pdbsum/1vy8 PDBsum]</span></td></tr> | ||
<table> | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clustered regularly interspaced short palindromic repeats (CRISPRs) are essential components of RNA-guided adaptive immune systems that protect bacteria and archaea from viruses and plasmids. In Escherichia coli, short CRISPR-derived RNAs (crRNAs) assemble into a 405 kDa multi-subunit surveillance complex called Cascade (CRISPR-associated complex for antiviral defense). Here, we present the 3.24 A resolution x-ray crystal structure of Cascade. Eleven proteins and a 61-nucleotide crRNA assemble into a sea-horse-shaped architecture that binds double-stranded DNA targets complementary to the crRNA-guide sequence. Conserved sequences on the 3'- and 5'-ends of the crRNA are anchored by proteins at opposite ends of the complex, while the guide sequence is displayed along a helical assembly of six interwoven subunits that present 5-nucleotide segments of the crRNA in pseudo A-form configuration. The structure of Cascade suggests a mechanism for assembly and provides insights into the mechanisms of target recognition. | ||
| + | |||
| + | Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli.,Jackson RN, Golden SM, van Erp PB, Carter J, Westra ER, Brouns SJ, van der Oost J, Terwilliger TC, Read RJ, Wiedenheft B Science. 2014 Aug 7. pii: 1256328. PMID:25103409<ref>PMID:25103409</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 05:50, 27 August 2014
Crystal structure of the E. coli CRISPR RNA-guided surveillance complex, Cascade
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