3wx4
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wx4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WX4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3wx4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WX4 FirstGlance]. <br> | ||
| - | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wx4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wx4 RCSB], [http://www.ebi.ac.uk/pdbsum/3wx4 PDBsum]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wx4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wx4 RCSB], [http://www.ebi.ac.uk/pdbsum/3wx4 PDBsum]</span></td></tr> |
| - | <table> | + | </table> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The T4 phage protein Arn (Anti restriction nuclease) was identified as an inhibitor of the restriction enzyme McrBC. However, until now its molecular mechanism remained unclear. In the present study we used structural approaches to investigate biological properties of Arn. A structural analysis of Arn revealed that its shape and negative charge distribution are similar to dsDNA, suggesting that this protein could act as a DNA mimic. In a subsequent proteomic analysis, we found that the bacterial histone-like protein H-NS interacts with Arn, implying a new function. An electrophoretic mobility shift assay showed that Arn prevents H-NS from binding to the Escherichia coli hns and T4 p8.1 promoters. In vitro gene expression and electron microscopy analyses also indicated that Arn counteracts the gene-silencing effect of H-NS on a reporter gene. Because McrBC and H-NS both participate in the host defense system, our findings suggest that T4 Arn might knock down these mechanisms using its DNA mimicking properties. | ||
| + | |||
| + | The T4 phage DNA mimic protein Arn inhibits the DNA binding activity of the bacterial histone-like protein H-NS.,Ho CH, Wang HC, Ko TP, Chang YC, Wang AH J Biol Chem. 2014 Sep 26;289(39):27046-54. doi: 10.1074/jbc.M114.590851. Epub, 2014 Aug 12. PMID:25118281<ref>PMID:25118281</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Ho, C H | + | [[Category: Ho, C H]] |
| - | [[Category: Ko, T P | + | [[Category: Ko, T P]] |
| - | [[Category: Wang, A H.J | + | [[Category: Wang, A H.J]] |
| - | [[Category: Wang, H C | + | [[Category: Wang, H C]] |
[[Category: Dna mimic]] | [[Category: Dna mimic]] | ||
[[Category: Gene regulation]] | [[Category: Gene regulation]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
Revision as of 12:10, 10 December 2014
CRYSTAL STRUCTURE of T4 PHAGE ARN PROTEIN
| |||||||||||
