1du7
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2tct|2TCT]], [[2trt|2TRT]], [[1a6i|1A6I]], [[1bj0|1BJ0]], [[1bjy|1BJY]], [[1bjz|1BJZ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1du7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1du7 OCA], [http://www.ebi.ac.uk/pdbsum/1du7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1du7 RCSB]</span> | ||
}} | }} | ||
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[[Category: Orth, P.]] | [[Category: Orth, P.]] | ||
[[Category: Saenger, W.]] | [[Category: Saenger, W.]] | ||
| - | [[Category: CTC]] | ||
[[Category: dna-binding protein]] | [[Category: dna-binding protein]] | ||
[[Category: hth-motif]] | [[Category: hth-motif]] | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:48:48 2008'' |
Revision as of 16:48, 30 March 2008
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| , resolution 2.51Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 2TCT, 2TRT, 1A6I, 1BJ0, 1BJY, 1BJZ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF TET REPRESSOR CLASS D WITH 4-EPI-TETRACYCLINE
Overview
The tetracycline repressor (TetR) regulates the most abundant resistance mechanism against the antibiotic tetracycline in grain-negative bacteria. The TetR protein and its mutants are commonly used as control elements to regulate gene expression in higher eukaryotes. We present the crystal structure of the TetR homodimer in complex with its palindromic DNA operator at 2.5 A resolution. Comparison to the structure of TetR in complex with the inducer tetracycline-Mg2+ allows the mechanism of induction to be deduced. Inducer binding in the repressor core initiates conformational changes starting with C-terminal unwinding and shifting of the short helix a6 in each monomer. This forces a pendulum-like motion of helix a4, which increases the separation of the attached DNA binding domains by 3 A, abolishing the affinity of TetR for its operator DNA.
About this Structure
1DU7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system., Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W, Nat Struct Biol. 2000 Mar;7(3):215-9. PMID:10700280
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