|
|
| Line 1: |
Line 1: |
| - | ==Crystal structure of the E. coli CRISPR RNA-guided surveillance complex, Cascade==
| + | #REDIRECT [[4tvx]] This PDB entry is obsolete and replaced by 4tvx |
| - | <StructureSection load='1vy8' size='340' side='right' caption='[[1vy8]], [[Resolution|resolution]] 3.24Å' scene=''>
| + | |
| - | == Structural highlights ==
| + | |
| - | <table><tr><td colspan='2'>[[1vy8]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VY8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VY8 FirstGlance]. <br>
| + | |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=23G:GUANOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>23G</scene></td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tvx|4tvx]], [[1vy9|1vy9]]</td></tr>
| + | |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vy8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vy8 RCSB], [http://www.ebi.ac.uk/pdbsum/1vy8 PDBsum]</span></td></tr>
| + | |
| - | <table>
| + | |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Clustered regularly interspaced short palindromic repeats (CRISPRs) are essential components of RNA-guided adaptive immune systems that protect bacteria and archaea from viruses and plasmids. In Escherichia coli, short CRISPR-derived RNAs (crRNAs) assemble into a 405 kDa multi-subunit surveillance complex called Cascade (CRISPR-associated complex for antiviral defense). Here, we present the 3.24 A resolution x-ray crystal structure of Cascade. Eleven proteins and a 61-nucleotide crRNA assemble into a sea-horse-shaped architecture that binds double-stranded DNA targets complementary to the crRNA-guide sequence. Conserved sequences on the 3'- and 5'-ends of the crRNA are anchored by proteins at opposite ends of the complex, while the guide sequence is displayed along a helical assembly of six interwoven subunits that present 5-nucleotide segments of the crRNA in pseudo A-form configuration. The structure of Cascade suggests a mechanism for assembly and provides insights into the mechanisms of target recognition.
| + | |
| - | | + | |
| - | Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli.,Jackson RN, Golden SM, van Erp PB, Carter J, Westra ER, Brouns SJ, van der Oost J, Terwilliger TC, Read RJ, Wiedenheft B Science. 2014 Aug 7. pii: 1256328. PMID:25103409<ref>PMID:25103409</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| - | __TOC__
| + | |
| - | </StructureSection>
| + | |
| - | [[Category: Carter, J.]]
| + | |
| - | [[Category: Golden, S M.]]
| + | |
| - | [[Category: Jackson, R N.]]
| + | |
| - | [[Category: Wiedenheft, B.]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Crispr]]
| + | |
| - | [[Category: Hydrolase-rna complex]]
| + | |
| - | [[Category: Rna]]
| + | |
| - | [[Category: Surveillance]]
| + | |
