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| - | ==Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome==
| + | #REDIRECT [[1vvj]] This PDB entry is obsolete and replaced by 1vvj |
| - | <StructureSection load='4kwz' size='340' side='right' caption='[[4kwz]], [[Resolution|resolution]] 3.44Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[4kwz]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KWZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KWZ FirstGlance]. <br>
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| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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| - | <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=1MG:1N-METHYLGUANOSINE-5-MONOPHOSPHATE'>1MG</scene></td></tr>
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| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kx0|4kx0]], [[4kx1|4kx1]], [[4kx2|4kx2]]</td></tr>
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| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kwz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kwz RCSB], [http://www.ebi.ac.uk/pdbsum/4kwz PDBsum]</span></td></tr>
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| - | <table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6 and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.
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| - | Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Escherichia coli]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Dunham, C M.]]
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| - | [[Category: Dunkle, J A.]]
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| - | [[Category: Maehigashi, T.]]
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| - | [[Category: 16]]
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| - | [[Category: 23]]
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| - | [[Category: 30]]
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| - | [[Category: 70]]
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| - | [[Category: Protein biosynthesis]]
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| - | [[Category: Ribosomal subunit]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Rna]]
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| - | [[Category: Transfer rna]]
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| - | [[Category: Trna]]
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