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4qjs
From Proteopedia
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| - | ''' | + | ==Crystal structure of the Thermus thermophilus 70S ribosome bound with Elongation Factor 4, 50S subunit of the 70S ribosome== |
| + | <StructureSection load='4qjs' size='340' side='right' caption='[[4qjs]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4qjs]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QJS FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qjt|4qjt]], [[4w2e|4w2e]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qjs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qjs RCSB], [http://www.ebi.ac.uk/pdbsum/4qjs PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Elongation factor 4 (EF4/LepA) is a highly conserved guanosine triphosphatase translation factor. It was shown to promote back-translocation of tRNAs on posttranslocational ribosome complexes and to compete with elongation factor G for interaction with pretranslocational ribosomes, inhibiting the elongation phase of protein synthesis. Here, we report a crystal structure of EF4-guanosine diphosphate bound to the Thermus thermophilus ribosome with a P-site tRNA at 2.9 angstroms resolution. The C-terminal domain of EF4 reaches into the peptidyl transferase center and interacts with the acceptor stem of the peptidyl-tRNA in the P site. The ribosome is in an unusual state of ratcheting with the 30S subunit rotated clockwise relative to the 50S subunit, resulting in a remodeled decoding center. The structure is consistent with EF4 functioning either as a back-translocase or a ribosome sequester. | ||
| - | + | Ribosome structure. Crystal structure of elongation factor 4 bound to a clockwise ratcheted ribosome.,Gagnon MG, Lin J, Bulkley D, Steitz TA Science. 2014 Aug 8;345(6197):684-7. doi: 10.1126/science.1253525. PMID:25104389<ref>PMID:25104389</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Thermus thermophilus]] | ||
| + | [[Category: Gagnon, M G.]] | ||
| + | [[Category: Lin, J.]] | ||
| + | [[Category: Steitz, T A.]] | ||
| + | [[Category: Acylated p-site trna]] | ||
| + | [[Category: Ef4]] | ||
| + | [[Category: Elogation]] | ||
| + | [[Category: Lepa]] | ||
| + | [[Category: Ribosome]] | ||
| + | [[Category: Translation]] | ||
Revision as of 00:11, 2 October 2014
Crystal structure of the Thermus thermophilus 70S ribosome bound with Elongation Factor 4, 50S subunit of the 70S ribosome
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Categories: Thermus thermophilus | Gagnon, M G. | Lin, J. | Steitz, T A. | Acylated p-site trna | Ef4 | Elogation | Lepa | Ribosome | Translation
