This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ej1
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1ej1 |SIZE=350|CAPTION= <scene name='initialview01'>1ej1</scene>, resolution 2.2Å | |PDB= 1ej1 |SIZE=350|CAPTION= <scene name='initialview01'>1ej1</scene>, resolution 2.2Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5 | + | |LIGAND= <scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE'>M7G</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| Line 29: | Line 29: | ||
[[Category: protein 5' mrna cap complex]] | [[Category: protein 5' mrna cap complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 11:39:47 2008'' |
Revision as of 09:39, 23 March 2008
| |||||||
| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COCRYSTAL STRUCTURE OF THE MESSENGER RNA 5' CAP-BINDING PROTEIN (EIF4E) BOUND TO 7-METHYL-GDP
Overview
The X-ray structure of the eukaryotic translation initiation factor 4E (eIF4E), bound to 7-methyl-GDP, has been determined at 2.2 A resolution. eIF4E recognizes 5' 7-methyl-G(5')ppp(5')N mRNA caps during the rate-limiting initiation step of translation. The protein resembles a cupped hand and consists of a curved, 8-stranded antiparallel beta sheet, backed by three long alpha helices. 7-methyl-GDP binds in a narrow cap-binding slot on the molecule's concave surface, where 7-methyl-guanine recognition is mediated by base sandwiching between two conserved tryptophans, plus formation of three hydrogen bonds and a van der Waals contact between its N7-methyl group and a third conserved tryptophan. The convex dorsal surface of the molecule displays a phylogenetically conserved hydrophobic/acidic portion, which may interact with other translation initiation factors and regulatory proteins.
About this Structure
1EJ1 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP., Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK, Cell. 1997 Jun 13;89(6):951-61. PMID:9200613
Page seeded by OCA on Sun Mar 23 11:39:47 2008
