1ewj
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1ewj |SIZE=350|CAPTION= <scene name='initialview01'>1ewj</scene>, resolution 2.50Å | |PDB= 1ewj |SIZE=350|CAPTION= <scene name='initialview01'>1ewj</scene>, resolution 2.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=BLM:BLEOMYCIN A2'>BLM</scene> | + | |LIGAND= <scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= TRANSPOSON TN5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae]) | |GENE= TRANSPOSON TN5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1qto|1QTO]], [[1byl|1BYL]], [[1ecs|1ECS]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ewj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ewj OCA], [http://www.ebi.ac.uk/pdbsum/1ewj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ewj RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Matoba, Y.]] | [[Category: Matoba, Y.]] | ||
[[Category: Sugiyama, M.]] | [[Category: Sugiyama, M.]] | ||
| - | [[Category: BLM]] | ||
[[Category: antibiotics resistance]] | [[Category: antibiotics resistance]] | ||
[[Category: bleomycin]] | [[Category: bleomycin]] | ||
| Line 33: | Line 35: | ||
[[Category: homodimer]] | [[Category: homodimer]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:10:58 2008'' |
Revision as of 17:10, 30 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | TRANSPOSON TN5 (Klebsiella pneumoniae) | ||||||
| Related: | 1QTO, 1BYL, 1ECS
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF BLEOMYCIN-BINDING PROTEIN COMPLEXED WITH BLEOMYCIN
Overview
The transposon Tn5 carries a gene designated ble that confers resistance to bleomycin (Bm). In this study, we determined the x-ray crystal structures of the ble gene product, designated BLMT, uncomplexed and complexed with Bm at 1.7 and 2.5 A resolution, respectively. The structure of BLMT is a dimer with two Bm-binding pockets composed of two large concavities and two long grooves. This crystal structure of BLMT complexed with Bm gives a precise mode for binding of the antibiotic to BLMT. The conformational change of BLMT generated by binding to Bm occurs at a beta-turn composed of the residues from Gln(97) to Thr(102). Crystallographic analysis of Bm bound to BLMT shows that two thiazolium rings of the bithiazole moiety are in the trans conformation. The axial ligand, which binds a metal ion, seems to be the primary amine in the beta-aminoalanine moiety. This report, which is the first with regard to the x-ray crystal structure of Bm, shows that the bithiazole moiety of Bm is far from the metal-binding domain. That is, Bm complexed with BLMT takes a more extended form than the drug complexed with DNA.
About this Structure
1EWJ is a Single protein structure of sequence from Klebsiella pneumoniae. Full crystallographic information is available from OCA.
Reference
Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin., Maruyama M, Kumagai T, Matoba Y, Hayashida M, Fujii T, Hata Y, Sugiyama M, J Biol Chem. 2001 Mar 30;276(13):9992-9. Epub 2000 Dec 29. PMID:11134052
Page seeded by OCA on Sun Mar 30 20:10:58 2008
