1f08
From Proteopedia
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|PDB= 1f08 |SIZE=350|CAPTION= <scene name='initialview01'>1f08</scene>, resolution 1.90Å | |PDB= 1f08 |SIZE=350|CAPTION= <scene name='initialview01'>1f08</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=BR:BROMIDE ION'>BR</scene> | + | |LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f08 OCA], [http://www.ebi.ac.uk/pdbsum/1f08 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f08 RCSB]</span> | ||
}} | }} | ||
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[[Category: Stenlund, A.]] | [[Category: Stenlund, A.]] | ||
[[Category: Vaughn, D E.]] | [[Category: Vaughn, D E.]] | ||
| - | [[Category: BR]] | ||
[[Category: dna-binding domain]] | [[Category: dna-binding domain]] | ||
[[Category: helicase]] | [[Category: helicase]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:13:14 2008'' |
Revision as of 17:13, 30 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS
Overview
Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure of the E1 DNA binding domain refined to 1.9 A resolution. Residues critical for DNA binding are located on an extended loop and an alpha helix. We identify the E1 dimerization surface by selective mutations at an E1/E1 interface observed in the crystal and propose a model for the (E1)2-DNA complex. These and other observations suggest how the E1 DNA binding domain orchestrates assembly of the hexameric helicase on the ori.
About this Structure
1F08 is a Single protein structure of sequence from Bovine papillomavirus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus., Enemark EJ, Chen G, Vaughn DE, Stenlund A, Joshua-Tor L, Mol Cell. 2000 Jul;6(1):149-58. PMID:10949036
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