1fk8

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|SITE=
|SITE=
|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/3-alpha-hydroxysteroid_dehydrogenase_(B-specific) 3-alpha-hydroxysteroid dehydrogenase (B-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.50 1.1.1.50]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-alpha-hydroxysteroid_dehydrogenase_(B-specific) 3-alpha-hydroxysteroid dehydrogenase (B-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.50 1.1.1.50] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=[[1fjh|1FJH]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fk8 OCA], [http://www.ebi.ac.uk/pdbsum/1fk8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fk8 RCSB]</span>
}}
}}
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[[Category: Maser, E.]]
[[Category: Maser, E.]]
[[Category: Reuter, K.]]
[[Category: Reuter, K.]]
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[[Category: NAD]]
 
[[Category: carbonyl reductase]]
[[Category: carbonyl reductase]]
[[Category: comamonas testosteroni]]
[[Category: comamonas testosteroni]]
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[[Category: xenobiotic]]
[[Category: xenobiotic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:10:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:24:41 2008''

Revision as of 17:24, 30 March 2008

Image:1fk8.jpg


PDB ID 1fk8

Drag the structure with the mouse to rotate
, resolution 1.95Å
Ligands:
Activity: 3-alpha-hydroxysteroid dehydrogenase (B-specific), with EC number 1.1.1.50
Related: 1FJH


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX WITH NAD OF 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE FROM COMAMONAS TESTOSTERONI, A MEMBER OF THE SHORT CHAIN DEHYDROGENASE/REDUCTASE FAMILY


Overview

The crystal structure of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni (3alpha-HSDH) as well as the structure of its binary complex with NAD(+) have been solved at 1.68-A and 1.95-A resolution, respectively. The enzyme is a member of the short chain dehydrogenase/reductase (SDR) family. Accordingly, the active center and the conformation of the bound nucleotide cofactor closely resemble those of other SDRs. The crystal structure reveals one homodimer per asymmetric unit representing the physiologically active unity. Dimerization takes place via an interface essentially built-up by helix alphaG and strand betaG of each subunit. So far this type of intermolecular contact has exclusively been observed in homotetrameric SDRs but never in the structure of a homodimeric SDR. The formation of a tetramer is blocked in 3alpha-HSDH by the presence of a predominantly alpha-helical subdomain which is missing in all other SDRs of known structure.

About this Structure

1FK8 is a Single protein structure of sequence from Comamonas testosteroni. Full crystallographic information is available from OCA.

Reference

The crystal structure of 3alpha -hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni shows a novel oligomerization pattern within the short chain dehydrogenase/reductase family., Grimm C, Maser E, Mobus E, Klebe G, Reuter K, Ficner R, J Biol Chem. 2000 Dec 29;275(52):41333-9. PMID:11007791

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