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2kb5

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Revision as of 19:25, 25 December 2014

Solution NMR Structure of Eosinophil Cationic Protein/RNase 3

Structural highlights

2kb5 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	RNASE3, ECP, RNS3 (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[ECP_HUMAN] Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Eosinophil cationic protein (ECP) / human RNase 3, a member of the RNase A family, is a remarkably cytotoxic protein implicated in asthma and allergies. These activites are probably due to ECP's ability to interact with and disrupt membranes and depend on two Trp, 19 Arg and possibly an extremely high conformational stability. Here, we have used NMR spectroscopy to assign essentially all (1)H, (15)N and backbone (13)C resonances, to solve the 3D structure in aqueous solution and to quantify its residue-level stability. The NMR solution structure was determined on the basis of 2316 distance constraints and is well-defined (backbone RMSD = 0.75A). The N-terminus and the loop composed of residues 114-123 are relatively well-ordered; in contrast, conformational diversity is observed for the loop segments 17-22, 65-68 and 92-95 and most exposed sidechains. The side chain NH groups of the two Trp and 19 Arg showed no significant protection against hydrogen/deuterium exchange. The most protected NH groups belong to the first and last two square-strands, and curiously, the first square-helix. Analysis of their exchange rates reveals a strikingly high global stability of 11.8 kcal/mol. This value and other stability measurements are used to better quantify ECP's unfolding thermodynamics. (c) 2009 Wiley Periodicals, Inc. Biopolymers, 2009.

"The (1)H, (13)C, (15)N resonance assignment, solution structure and residue level stability of eosinophil cationic protein/rnase 3 determined by NMR spectroscopy",Laurents DV, Bruix M, Jimenez MA, Santoro J, Boix E, Moussaoui M, Nogues MV, Rico M Biopolymers. 2009 Feb 2. PMID:19189375^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gabay JE, Scott RW, Campanelli D, Griffith J, Wilde C, Marra MN, Seeger M, Nathan CF. Antibiotic proteins of human polymorphonuclear leukocytes. Proc Natl Acad Sci U S A. 1989 Jul;86(14):5610-4. PMID:2501794
↑ Torrent M, de la Torre BG, Nogues VM, Andreu D, Boix E. Bactericidal and membrane disruption activities of the eosinophil cationic protein are largely retained in an N-terminal fragment. Biochem J. 2009 Jul 15;421(3):425-34. doi: 10.1042/BJ20082330. PMID:19450231 doi:10.1042/BJ20082330
↑ Laurents DV, Bruix M, Jimenez MA, Santoro J, Boix E, Moussaoui M, Nogues MV, Rico M. "The (1)H, (13)C, (15)N resonance assignment, solution structure and residue level stability of eosinophil cationic protein/rnase 3 determined by NMR spectroscopy" Biopolymers. 2009 Feb 2. PMID:19189375 doi:10.1002/bip.21152

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kb5"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2kb5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KB5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KB5 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RNASE3, ECP, RNS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RNASE3, ECP, RNS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kb5 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb5 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kb5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kb5 RCSB], [http://www.ebi.ac.uk/pdbsum/2kb5 PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/ECP_HUMAN ECP_HUMAN]] Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.<ref>PMID:2501794</ref> <ref>PMID:19450231</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 27: / Line 29: @@
 ==See Also==
 *[[Ribonuclease|Ribonuclease]]
+*[[Temp|Temp]]
+*[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]]
 == References ==
 <references/>
@@ Line 32: / Line 36: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Boix, E.]]
+[[Category: Boix, E]]
-[[Category: Bruix, M.]]
+[[Category: Bruix, M]]
-[[Category: Jimenez, M.]]
+[[Category: Jimenez, M]]
-[[Category: Laurents, D V.]]
+[[Category: Laurents, D V]]
-[[Category: Moussaoui, M.]]
+[[Category: Moussaoui, M]]
-[[Category: Nogues, M.]]
+[[Category: Nogues, M]]
-[[Category: Rico, M.]]
+[[Category: Rico, M]]
-[[Category: Santoro, J.]]
+[[Category: Santoro, J]]
 [[Category: Antibiotic]]
 [[Category: Antimicrobial]]

2kb5

From Proteopedia

Revision as of 19:25, 25 December 2014

Solution NMR Structure of Eosinophil Cationic Protein/RNase 3

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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