1fwx
From Proteopedia
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|PDB= 1fwx |SIZE=350|CAPTION= <scene name='initialview01'>1fwx</scene>, resolution 1.60Å | |PDB= 1fwx |SIZE=350|CAPTION= <scene name='initialview01'>1fwx</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=CUZ:(MU-4-SULFIDO)-TETRA-NUCLEAR+COPPER+ION'>CUZ</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrous-oxide_reductase Nitrous-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.6 1.7.99.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrous-oxide_reductase Nitrous-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.6 1.7.99.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1qni|1qni]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fwx OCA], [http://www.ebi.ac.uk/pdbsum/1fwx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fwx RCSB]</span> | ||
}} | }} | ||
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[[Category: Saraste, M.]] | [[Category: Saraste, M.]] | ||
[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: CUA]] | ||
| - | [[Category: CUZ]] | ||
[[Category: beta-propeller domain]] | [[Category: beta-propeller domain]] | ||
[[Category: cua site]] | [[Category: cua site]] | ||
| Line 41: | Line 40: | ||
[[Category: cuz site]] | [[Category: cuz site]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:31:51 2008'' |
Revision as of 17:31, 30 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Activity: | Nitrous-oxide reductase, with EC number 1.7.99.6 | ||||||
| Related: | 1qni
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS
Overview
Nitrous-oxide reductases (N2OR) catalyze the two-electron reduction of N(2)O to N(2). The crystal structure of N2ORs from Pseudomonas nautica (Pn) and Paracoccus denitrificans (Pd) were solved at resolutions of 2.4 and 1.6 A, respectively. The Pn N2OR structure revealed that the catalytic CuZ center belongs to a new type of metal cluster in which four copper ions are liganded by seven histidine residues. A bridging oxygen moiety and two other hydroxide ligands were proposed to complete the ligation scheme (Brown, K., Tegoni, M., Prudencio, M., Pereira, A. S., Besson, S., Moura, J. J. G., Moura, I., and Cambillau, C. (2000) Nat. Struct. Biol. 7, 191-195). However, in the CuZ cluster, inorganic sulfur chemical determination and the high resolution structure of Pd N2OR identified a bridging inorganic sulfur instead of an oxygen. This result reconciles the novel CuZ cluster with the hitherto puzzling spectroscopic data.
About this Structure
1FWX is a Single protein structure of sequence from Paracoccus denitrificans. Full crystallographic information is available from OCA.
Reference
Revisiting the catalytic CuZ cluster of nitrous oxide (N2O) reductase. Evidence of a bridging inorganic sulfur., Brown K, Djinovic-Carugo K, Haltia T, Cabrito I, Saraste M, Moura JJ, Moura I, Tegoni M, Cambillau C, J Biol Chem. 2000 Dec 29;275(52):41133-6. PMID:11024061
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