1g7t
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1g7t |SIZE=350|CAPTION= <scene name='initialview01'>1g7t</scene>, resolution 2.Å | |PDB= 1g7t |SIZE=350|CAPTION= <scene name='initialview01'>1g7t</scene>, resolution 2.Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1g7s|1G7S]], [[1g7r|1G7R]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g7t OCA], [http://www.ebi.ac.uk/pdbsum/1g7t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g7t RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Dever, T E.]] | [[Category: Dever, T E.]] | ||
[[Category: Roll-Mecak, A.]] | [[Category: Roll-Mecak, A.]] | ||
| - | [[Category: GNP]] | ||
| - | [[Category: MG]] | ||
[[Category: translational gtpase]] | [[Category: translational gtpase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:38:27 2008'' |
Revision as of 17:38, 30 March 2008
| |||||||
| , resolution 2.Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1G7S, 1G7R
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDPNP
Overview
X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes in all three kingdoms of life. The conserved core of IF2/eIF5B consists of an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a molecular lever, which amplifies a modest conformational change in the Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a distance of 90 A from the G domain active center to domain IV. Mechanisms of GTPase function and ribosome binding are discussed.
About this Structure
1G7T is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:11114334
Page seeded by OCA on Sun Mar 30 20:38:27 2008
