1g92
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g92 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g92 OCA], [http://www.ebi.ac.uk/pdbsum/1g92 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g92 RCSB]</span> | ||
}} | }} | ||
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[[Category: Poznanski, J.]] | [[Category: Poznanski, J.]] | ||
[[Category: Szolajska, E.]] | [[Category: Szolajska, E.]] | ||
| - | [[Category: | + | [[Category: neurotoxin]] |
| + | [[Category: sodium channel inhibitor]] | ||
| + | [[Category: toxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:39:12 2008'' |
Revision as of 17:39, 30 March 2008
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF PONERATOXIN
Overview
Poneratoxin is a small neuropeptide found in the venom of the ant Paraponera clavata. It is stored in the venom reservoir as an inactive 25-residue peptide. Here we describe both chemically synthesized poneratoxin and poneratoxin obtained by expression in insect cells. When expressed in insect cells, poneratoxin was observed attached to cell membranes. Both synthetic and recombinant ponerotoxins were soluble below pH 4.5. The structure of synthetic poneratoxin was characterized by circular dichroism and solved by nuclear magnetic resonance. In an environment imitating a lipid bilayer, at pH within the range of insect hemolymph, synthetic poneratoxin has a V shape, with two alpha-helices connected by a beta-turn. Insect larvae were paralyzed by injection of either of the purified toxins, with the recombinant one acting faster. The recombinant toxin-producing baculovirus reduced the average survival time of the insect host by 25 h compared with unmodified virus. Mass spectrometry analysis showed that the recombinant toxin has an N-terminal 21-residue extension, possibly improving its stability and/or stabilizing the membrane-bound state. The potential use of poneratoxin for the construction of biological insecticide is discussed.
About this Structure
1G92 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide., Szolajska E, Poznanski J, Ferber ML, Michalik J, Gout E, Fender P, Bailly I, Dublet B, Chroboczek J, Eur J Biochem. 2004 Jun;271(11):2127-36. PMID:15153103
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