1gwg
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1gwg |SIZE=350|CAPTION= <scene name='initialview01'>1gwg</scene>, resolution 2.01Å | |PDB= 1gwg |SIZE=350|CAPTION= <scene name='initialview01'>1gwg</scene>, resolution 2.01Å | ||
|SITE= <scene name='pdbsite=CD1:Cd+Binding+Site+For+Chain+A'>CD1</scene> | |SITE= <scene name='pdbsite=CD1:Cd+Binding+Site+For+Chain+A'>CD1</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwg OCA], [http://www.ebi.ac.uk/pdbsum/1gwg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gwg RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Bricogne, G.]] | [[Category: Bricogne, G.]] | ||
[[Category: Evans, G.]] | [[Category: Evans, G.]] | ||
| - | [[Category: CD]] | ||
| - | [[Category: IOD]] | ||
[[Category: acetylation]] | [[Category: acetylation]] | ||
[[Category: iron storage]] | [[Category: iron storage]] | ||
[[Category: multigene family]] | [[Category: multigene family]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:52:43 2008'' |
Revision as of 17:52, 30 March 2008
| |||||||
| , resolution 2.01Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRI-IODIDE DERIVATIVE OF APOFERRITIN
Overview
A series of experiments performed at Cu Kalpha wavelength on in-house X-ray equipment are presented which investigate two possibilities for enhancing the experimental phasing signal by means of (i) triiodide/iodide soaks using KI/I(2) and (ii) combinations of counter-ions introduced using the short cryosoak method. Triiodide-derivative crystal structures for five test proteins have been refined and reveal that iodine can bind as polyiodide and single iodide ions through hydrophobic and hydrogen-bonding interactions both at the molecular surface and in intramolecular and intermolecular cavities. In three cases, the structures could be automatically determined with autoSHARP using in-house SAD and SIRAS data. The investigation of combinatorial counter-ion replacement using multiple salts with Na(+) and Cs(+) as cations and I(-) and Cl(-) as anions reveals that, for the case of hen egg-white lysozyme, significant improvement in phasing signal is obtained by the combined use of salts compared with SIRAS methods using native and single short-soak derivative data sets.
About this Structure
1GWG is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300
Page seeded by OCA on Sun Mar 30 20:52:43 2008
