1s1e

From Proteopedia

(Difference between revisions)

Revision as of 18:21, 25 December 2014

Crystal Structure of Kv Channel-interacting protein 1 (KChIP-1)

Structural highlights

1s1e is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	KCNIP1 (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[KCIP1_HUMAN] Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Seems to be involved in KCND2 trafficking to the cell surface.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The family of calcium binding proteins called KChIPs associates with Kv4 family K(+) channels and modulates their biophysical properties. Here, using mutagenesis and X-ray crystallography, we explore the interaction between Kv4 subunits and KChIP1. Two regions in the Kv4.2 N terminus, residues 7-11 and 71-90, are necessary for KChIP1 modulation and interaction with Kv4.2. When inserted into the Kv1.2 N terminus, residues 71-90 of Kv4.2 are also sufficient to confer association with KChIP1. To provide a structural framework for these data, we solved the crystal structures of Kv4.3N and KChIP1 individually. Taken together with the mutagenesis data, the individual structures suggest that that the Kv4 N terminus is required for stable association with KChIP1, perhaps through a hydrophobic surface interaction, and that residues 71-90 in Kv4 subunits form a contact loop that mediates the specific association of KChIPs with Kv4 subunits.

Two N-terminal domains of Kv4 K(+) channels regulate binding to and modulation by KChIP1.,Scannevin RH, Wang K, Jow F, Megules J, Kopsco DC, Edris W, Carroll KC, Lu Q, Xu W, Xu Z, Katz AH, Olland S, Lin L, Taylor M, Stahl M, Malakian K, Somers W, Mosyak L, Bowlby MR, Chanda P, Rhodes KJ Neuron. 2004 Feb 19;41(4):587-98. PMID:14980207^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ An WF, Bowlby MR, Betty M, Cao J, Ling HP, Mendoza G, Hinson JW, Mattsson KI, Strassle BW, Trimmer JS, Rhodes KJ. Modulation of A-type potassium channels by a family of calcium sensors. Nature. 2000 Feb 3;403(6769):553-6. PMID:10676964 doi:10.1038/35000592
↑ Nakamura TY, Nandi S, Pountney DJ, Artman M, Rudy B, Coetzee WA. Different effects of the Ca(2+)-binding protein, KChIP1, on two Kv4 subfamily members, Kv4.1 and Kv4.2. FEBS Lett. 2001 Jun 22;499(3):205-9. PMID:11423117
↑ Shibata R, Misonou H, Campomanes CR, Anderson AE, Schrader LA, Doliveira LC, Carroll KI, Sweatt JD, Rhodes KJ, Trimmer JS. A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels. J Biol Chem. 2003 Sep 19;278(38):36445-54. Epub 2003 Jun 26. PMID:12829703 doi:10.1074/jbc.M306142200
↑ Scannevin RH, Wang K, Jow F, Megules J, Kopsco DC, Edris W, Carroll KC, Lu Q, Xu W, Xu Z, Katz AH, Olland S, Lin L, Taylor M, Stahl M, Malakian K, Somers W, Mosyak L, Bowlby MR, Chanda P, Rhodes KJ. Two N-terminal domains of Kv4 K(+) channels regulate binding to and modulation by KChIP1. Neuron. 2004 Feb 19;41(4):587-98. PMID:14980207

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1s1e"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1s1e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S1E FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KCNIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KCNIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s1e RCSB], [http://www.ebi.ac.uk/pdbsum/1s1e PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s1e RCSB], [http://www.ebi.ac.uk/pdbsum/1s1e PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/KCIP1_HUMAN KCIP1_HUMAN]] Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Seems to be involved in KCND2 trafficking to the cell surface.<ref>PMID:10676964</ref> <ref>PMID:11423117</ref> <ref>PMID:12829703</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 32: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Bowlby, M R.]]
+[[Category: Bowlby, M R]]
-[[Category: Carroll, K C.]]
+[[Category: Carroll, K C]]
-[[Category: Chanda, P.]]
+[[Category: Chanda, P]]
-[[Category: Edris, W.]]
+[[Category: Edris, W]]
-[[Category: Jow, F.]]
+[[Category: Jow, F]]
-[[Category: Katz, A H.]]
+[[Category: Katz, A H]]
-[[Category: Kopsco, D C.]]
+[[Category: Kopsco, D C]]
-[[Category: Lin, L.]]
+[[Category: Lin, L]]
-[[Category: Lu, Q.]]
+[[Category: Lu, Q]]
-[[Category: Malakian, K.]]
+[[Category: Malakian, K]]
-[[Category: Megules, J.]]
+[[Category: Megules, J]]
-[[Category: Mosyak, L.]]
+[[Category: Mosyak, L]]
-[[Category: Olland, S.]]
+[[Category: Olland, S]]
-[[Category: Rhodes, K J.]]
+[[Category: Rhodes, K J]]
-[[Category: Scannevin, R H.]]
+[[Category: Scannevin, R H]]
-[[Category: Somers, W.]]
+[[Category: Somers, W]]
-[[Category: Stahl, M.]]
+[[Category: Stahl, M]]
-[[Category: Taylor, M.]]
+[[Category: Taylor, M]]
-[[Category: Wang, K W.]]
+[[Category: Wang, K W]]
-[[Category: Xu, W X.]]
+[[Category: Xu, W X]]
-[[Category: Xu, Z B.]]
+[[Category: Xu, Z B]]
 [[Category: Calcium-binding protein]]
 [[Category: Ef-finger]]

1s1e

From Proteopedia

Revision as of 18:21, 25 December 2014

Crystal Structure of Kv Channel-interacting protein 1 (KChIP-1)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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