1y7i

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Revision as of 21:37, 25 December 2014

Structural and biochemical studies identify tobacco SABP2 as a methylsalicylate esterase and further implicate it in plant innate immunity, Northeast Structural Genomics Target AR2241

Structural highlights

1y7i is a 2 chain structure with sequence from Nicotiana tabacum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Related:	1xkl, 1y7h
Resources:	FirstGlance, OCA, RCSB, PDBsum, TOPSAN

Function

[Q6RYA0_TOBAC] Required to convert methyl salicylate (MeSA) to salicylic acid (SA) as part of the signal transduction pathways that activate systemic acquired resistance in systemic tissue. MeSA is believed to be an inactive form that needs to be demethylated to exert a biological effect. Also able to catalyze the conversion of acibenzolar-S-methyl into acibenzolar to induce systemic acquired resistance.^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Salicylic acid (SA) is a critical signal for the activation of plant defense responses against pathogen infections. We recently identified SA-binding protein 2 (SABP2) from tobacco as a protein that displays high affinity for SA and plays a crucial role in the activation of systemic acquired resistance to plant pathogens. Here we report the crystal structures of SABP2, alone and in complex with SA at up to 2.1-A resolution. The structures confirm that SABP2 is a member of the alpha/beta hydrolase superfamily of enzymes, with Ser-81, His-238, and Asp-210 as the catalytic triad. SA is bound in the active site and is completely shielded from the solvent, consistent with the high affinity of this compound for SABP2. Our biochemical studies reveal that SABP2 has strong esterase activity with methyl salicylate as the substrate, and that SA is a potent product inhibitor of this catalysis. Modeling of SABP2 with MeSA in the active site is consistent with all these biochemical observations. Our results suggest that SABP2 may be required to convert MeSA to SA as part of the signal transduction pathways that activate systemic acquired resistance and perhaps local defense responses as well.

Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity.,Forouhar F, Yang Y, Kumar D, Chen Y, Fridman E, Park SW, Chiang Y, Acton TB, Montelione GT, Pichersky E, Klessig DF, Tong L Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1773-8. Epub 2005 Jan 24. PMID:15668381^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumar D, Klessig DF. High-affinity salicylic acid-binding protein 2 is required for plant innate immunity and has salicylic acid-stimulated lipase activity. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):16101-6. Epub 2003 Dec 12. PMID:14673096 doi:http://dx.doi.org/10.1073/pnas.0307162100
↑ Park SW, Kaimoyo E, Kumar D, Mosher S, Klessig DF. Methyl salicylate is a critical mobile signal for plant systemic acquired resistance. Science. 2007 Oct 5;318(5847):113-6. PMID:17916738 doi:http://dx.doi.org/10.1126/science.1147113
↑ Park SW, Liu PP, Forouhar F, Vlot AC, Tong L, Tietjen K, Klessig DF. Use of a synthetic salicylic acid analog to investigate the roles of methyl salicylate and its esterases in plant disease resistance. J Biol Chem. 2009 Mar 13;284(11):7307-17. doi: 10.1074/jbc.M807968200. Epub 2009 , Jan 8. PMID:19131332 doi:http://dx.doi.org/10.1074/jbc.M807968200
↑ Padhi SK, Fujii R, Legatt GA, Fossum SL, Berchtold R, Kazlauskas RJ. Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions. Chem Biol. 2010 Aug 27;17(8):863-71. doi: 10.1016/j.chembiol.2010.06.013. PMID:20797615 doi:http://dx.doi.org/10.1016/j.chembiol.2010.06.013
↑ Tripathi D, Jiang YL, Kumar D. SABP2, a methyl salicylate esterase is required for the systemic acquired resistance induced by acibenzolar-S-methyl in plants. FEBS Lett. 2010 Aug 4;584(15):3458-63. doi: 10.1016/j.febslet.2010.06.046. Epub, 2010 Jul 14. PMID:20621100 doi:http://dx.doi.org/10.1016/j.febslet.2010.06.046
↑ Forouhar F, Yang Y, Kumar D, Chen Y, Fridman E, Park SW, Chiang Y, Acton TB, Montelione GT, Pichersky E, Klessig DF, Tong L. Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1773-8. Epub 2005 Jan 24. PMID:15668381
↑ Forouhar F, Yang Y, Kumar D, Chen Y, Fridman E, Park SW, Chiang Y, Acton TB, Montelione GT, Pichersky E, Klessig DF, Tong L. Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1773-8. Epub 2005 Jan 24. PMID:15668381

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y7i"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1y7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Y7I FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene><br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xkl|1xkl]], [[1y7h|1y7h]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xkl|1xkl]], [[1y7h|1y7h]]</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y7i RCSB], [http://www.ebi.ac.uk/pdbsum/1y7i PDBsum], [http://www.topsan.org/Proteins/NESGC/1y7i TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1y7i RCSB], [http://www.ebi.ac.uk/pdbsum/1y7i PDBsum], [http://www.topsan.org/Proteins/NESGC/1y7i TOPSAN]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/Q6RYA0_TOBAC Q6RYA0_TOBAC]] Required to convert methyl salicylate (MeSA) to salicylic acid (SA) as part of the signal transduction pathways that activate systemic acquired resistance in systemic tissue. MeSA is believed to be an inactive form that needs to be demethylated to exert a biological effect. Also able to catalyze the conversion of acibenzolar-S-methyl into acibenzolar to induce systemic acquired resistance.<ref>PMID:14673096</ref> <ref>PMID:17916738</ref> <ref>PMID:19131332</ref> <ref>PMID:20797615</ref> <ref>PMID:20621100</ref> <ref>PMID:15668381</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 33: @@
 </StructureSection>
 [[Category: Nicotiana tabacum]]
-[[Category: Acton, T B.]]
+[[Category: Acton, T B]]
-[[Category: Chen, Y.]]
+[[Category: Chen, Y]]
-[[Category: Chiang, Y.]]
+[[Category: Chiang, Y]]
-[[Category: Forouhar, F.]]
+[[Category: Forouhar, F]]
-[[Category: Fridman, E.]]
+[[Category: Fridman, E]]
-[[Category: Klessig, D F.]]
+[[Category: Klessig, D F]]
-[[Category: Kumar, D.]]
+[[Category: Kumar, D]]
-[[Category: Montelione, G T.]]
+[[Category: Montelione, G T]]
-[[Category: NESG, Northeast Structural Genomics Consortium.]]
+[[Category: Structural genomic]]
-[[Category: Park, S W.]]
+[[Category: Park, S W]]
-[[Category: Pichersky, E.]]
+[[Category: Pichersky, E]]
-[[Category: Tong, L.]]
+[[Category: Tong, L]]
-[[Category: Yang, Y.]]
+[[Category: Yang, Y]]
 [[Category: Alpha-beta protein]]
 [[Category: Nesg]]
-[[Category: Northeast structural genomics consortium]]
+[[Category: PSI, Protein structure initiative]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Structural genomic]]
 [[Category: Unknown function]]

1y7i

From Proteopedia

Revision as of 21:37, 25 December 2014

Structural and biochemical studies identify tobacco SABP2 as a methylsalicylate esterase and further implicate it in plant innate immunity, Northeast Structural Genomics Target AR2241

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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