1hjk
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
|PDB= 1hjk |SIZE=350|CAPTION= <scene name='initialview01'>1hjk</scene>, resolution 2.3Å | |PDB= 1hjk |SIZE=350|CAPTION= <scene name='initialview01'>1hjk</scene>, resolution 2.3Å | ||
|SITE= <scene name='pdbsite=CTA:Catalytically+Active+Site+On+A+Subunit'>CTA</scene> and <scene name='pdbsite=CTB:Catalytically+Active+Site+On+B+Subunit'>CTB</scene> | |SITE= <scene name='pdbsite=CTA:Catalytically+Active+Site+On+A+Subunit'>CTA</scene> and <scene name='pdbsite=CTB:Catalytically+Active+Site+On+B+Subunit'>CTB</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span> |
|GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= PHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjk OCA], [http://www.ebi.ac.uk/pdbsum/1hjk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hjk RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Murphy, J E.]] | [[Category: Murphy, J E.]] | ||
[[Category: Stec, B.]] | [[Category: Stec, B.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: ZN]] | ||
[[Category: alcohol acceptor)]] | [[Category: alcohol acceptor)]] | ||
[[Category: alkaline phosphatase]] | [[Category: alkaline phosphatase]] | ||
| Line 36: | Line 36: | ||
[[Category: transferase(phospho]] | [[Category: transferase(phospho]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:08 2008'' |
Revision as of 18:06, 30 March 2008
| |||||||
| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | , , , | ||||||
| Gene: | PHOA (Escherichia coli) | ||||||
| Activity: | Alkaline phosphatase, with EC number 3.1.3.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ALKALINE PHOSPHATASE MUTANT H331Q
Overview
Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.
About this Structure
1HJK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:9253408
Page seeded by OCA on Sun Mar 30 21:06:08 2008
