1hkz
From Proteopedia
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|PDB= 1hkz |SIZE=350|CAPTION= <scene name='initialview01'>1hkz</scene>, resolution 2.49Å | |PDB= 1hkz |SIZE=350|CAPTION= <scene name='initialview01'>1hkz</scene>, resolution 2.49Å | ||
|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=C1O:CU-O+LINKAGE'>C1O</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkz OCA], [http://www.ebi.ac.uk/pdbsum/1hkz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hkz RCSB]</span> | ||
}} | }} | ||
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[[Category: Marcal, D.]] | [[Category: Marcal, D.]] | ||
[[Category: Martins, L O.]] | [[Category: Martins, L O.]] | ||
| - | [[Category: C1O]] | ||
| - | [[Category: CU]] | ||
| - | [[Category: CU1]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: copper-binding]] | ||
[[Category: endospore coat]] | [[Category: endospore coat]] | ||
[[Category: laccase]] | [[Category: laccase]] | ||
| - | [[Category: oxidase]] | + | [[Category: oxidase,copper-binding]] |
| - | [[Category: reaction cycle | + | [[Category: reaction cycle,sporulation]] |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:07:02 2008'' |
Revision as of 18:07, 30 March 2008
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| , resolution 2.49Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF BACILLUS SUBTILIS COTA AFTER 8H SOAKING WITH ABTS
Overview
The CotA laccase from the endospore coat of Bacillus subtilis has been crystallized in the presence of the non-catalytic co-oxidant 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and the structure was determined using synchrotron radiation. The binding site for this adduct is well defined and indicates how ABTS, in conjunction with laccases, could act as an oxidative mediator toward non-phenolic moieties. In addition, a dioxygen moiety is clearly defined within the solvent channel oriented toward one of the T3 copper atoms in the trinuclear center.
About this Structure
1HKZ is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis., Enguita FJ, Marcal D, Martins LO, Grenha R, Henriques AO, Lindley PF, Carrondo MA, J Biol Chem. 2004 May 28;279(22):23472-6. Epub 2004 Feb 4. PMID:14764581
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