2cse
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2cse]] is a 27 chain structure with sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_1_lang Mammalian orthoreovirus 1 lang] and [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_dearing Mammalian orthoreovirus 3 dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CSE FirstGlance]. <br> | <table><tr><td colspan='2'>[[2cse]] is a 27 chain structure with sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_1_lang Mammalian orthoreovirus 1 lang] and [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_dearing Mammalian orthoreovirus 3 dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CSE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CSE FirstGlance]. <br> | ||
| - | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cse RCSB], [http://www.ebi.ac.uk/pdbsum/2cse PDBsum]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cse OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2cse RCSB], [http://www.ebi.ac.uk/pdbsum/2cse PDBsum]</span></td></tr> |
| - | <table> | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/LMBD1_REOVL LMBD1_REOVL]] Inner capsid (core) component. Displays NTPase, RNA 5'-triphosphatase (RTPase) and RNA helicase activities and probably participates in transcription of the viral genome. Helicase activity might be involved in unwinding or reannealing dsRNA during RNA synthesis. RTPase enzymatic activity represents the first step in RNA capping, which yields a 5'-diphosphorylated plus-strand RNA (By similarity). [[http://www.uniprot.org/uniprot/MU1_REOVL MU1_REOVL]] Major outer capsid protein involved in host cell membrane penetration. In the endocytic compartment, outer-capsid protein sigma-3 is removed by cathepsin proteases, which exposes the viral membrane-penetration protein mu-1. Both myristoylated peptides mu-1N and phi are released during infectious subvirion particles (ISVP) formation in the endosome. They associate with host membranes and mu-1N induces permeabilization and delivery of transcriptionally active viral particles into the host cell cytoplasm. Seems to induce apoptosis in the host cell.<ref>PMID:11007773</ref> <ref>PMID:15280481</ref> <ref>PMID:16912293</ref> <ref>PMID:17005655</ref> <ref>PMID:18369316</ref> The viral outer shell polypeptides, of which mu-1 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3.<ref>PMID:11007773</ref> <ref>PMID:15280481</ref> <ref>PMID:16912293</ref> <ref>PMID:17005655</ref> <ref>PMID:18369316</ref> [[http://www.uniprot.org/uniprot/LMBD2_REOVD LMBD2_REOVD]] Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities. [[http://www.uniprot.org/uniprot/SIGM3_REOVL SIGM3_REOVL]] Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding (By similarity). The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3. [[http://www.uniprot.org/uniprot/SIGM2_REOVL SIGM2_REOVL]] Inner capsid (core) component. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Mammalian orthoreovirus 1 lang]] | [[Category: Mammalian orthoreovirus 1 lang]] | ||
[[Category: Mammalian orthoreovirus 3 dearing]] | [[Category: Mammalian orthoreovirus 3 dearing]] | ||
| - | [[Category: Baker, T S | + | [[Category: Baker, T S]] |
| - | [[Category: Harrison, S C | + | [[Category: Harrison, S C]] |
| - | [[Category: Ji, Y | + | [[Category: Ji, Y]] |
| - | [[Category: Marinescu, D C | + | [[Category: Marinescu, D C]] |
| - | [[Category: Nibert, M L | + | [[Category: Nibert, M L]] |
| - | [[Category: Zhang, L | + | [[Category: Zhang, L]] |
| - | [[Category: Zhang, X | + | [[Category: Zhang, X]] |
[[Category: Cryoem]] | [[Category: Cryoem]] | ||
[[Category: Icosahedral virus]] | [[Category: Icosahedral virus]] | ||
Revision as of 20:26, 25 December 2014
Features of Reovirus Outer-Capsid Protein mu1 Revealed by Electron and Image Reconstruction of the virion at 7.0-A Resolution
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