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2k85

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Revision as of 17:57, 25 December 2014

p190-A RhoGAP FF1 domain

Structural highlights

2k85 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	GRLF1, GRF1, KIAA1722 (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[GRLF1_HUMAN] Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

p190-A and -B Rho GAPs (guanosine triphosphatase activating proteins) are the only cytoplasmatic proteins containing FF domains. In p190-A Rho GAP, the region containing the FF domains has been implicated in binding to the transcription factor TFII-I. Moreover, phosphorylation of Tyr308 within the first FF domain inhibits this interaction. Because the structural determinants governing this mechanism remain unknown, we sought to solve the structure of the first FF domain of p190-A Rho GAP (RhoGAPFF1) and to study the potential impact of phosphorylation on the structure. We found that RhoGAPFF1 does not fold with the typical (alpha1-alpha2-3(10)-alpha 3) arrangement of other FF domains. Instead, the NMR data obtained at 285 K show an alpha1-alpha2-alpha 3-alpha 4 topology. In addition, we observed that specific contacts between residues in the first loop and the fourth helix are indispensable for the correct folding and stability of this domain. The structure also revealed that Tyr308 contributes to the domain hydrophobic core. Furthermore, the residues that compose the target motif of the platelet-derived growth factor receptor alpha kinase form part of the alpha 3 helix. We observed that the phosphorylation reaction requires a previous step including domain unfolding, a process that occurs at 310 K. In the absence of phosphorylation, the temperature-dependent RhoGAPFF1 folding/unfolding process is reversible. However, phosphorylation causes an irreversible destabilization of the RhoGAPFF1 structure, which probably accounts for the inhibitory effect that it exerts on the TFII-I interaction. Our results link the ability of a protein domain to be phosphorylated with conformational changes in its three-dimensional structure.

NMR structural studies on human p190-A RhoGAPFF1 revealed that domain phosphorylation by the PDGF-receptor alpha requires its previous unfolding.,Bonet R, Ruiz L, Aragon E, Martin-Malpartida P, Macias MJ J Mol Biol. 2009 Jun 5;389(2):230-7. Epub 2009 Apr 22. PMID:19393245^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ LeClerc S, Palaniswami R, Xie BX, Govindan MV. Molecular cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J Biol Chem. 1991 Sep 15;266(26):17333-40. PMID:1894621
↑ Tikoo A, Czekay S, Viars C, White S, Heath JK, Arden K, Maruta H. p190-A, a human tumor suppressor gene, maps to the chromosomal region 19q13.3 that is reportedly deleted in some gliomas. Gene. 2000 Oct 17;257(1):23-31. PMID:11054565
↑ Bonet R, Ruiz L, Aragon E, Martin-Malpartida P, Macias MJ. NMR structural studies on human p190-A RhoGAPFF1 revealed that domain phosphorylation by the PDGF-receptor alpha requires its previous unfolding. J Mol Biol. 2009 Jun 5;389(2):230-7. Epub 2009 Apr 22. PMID:19393245 doi:10.1016/j.jmb.2009.04.035

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2k85"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2k85]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K85 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K85 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GRLF1, GRF1, KIAA1722 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GRLF1, GRF1, KIAA1722 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k85 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k85 RCSB], [http://www.ebi.ac.uk/pdbsum/2k85 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k85 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k85 RCSB], [http://www.ebi.ac.uk/pdbsum/2k85 PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/GRLF1_HUMAN GRLF1_HUMAN]] Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.<ref>PMID:1894621</ref> <ref>PMID:11054565</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 31: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Bonet, R.]]
+[[Category: Bonet, R]]
-[[Category: Macias, M.]]
+[[Category: Macias, M]]
-[[Category: Martin-Malpartida, P.]]
+[[Category: Martin-Malpartida, P]]
-[[Category: Ruiz, L.]]
+[[Category: Ruiz, L]]
 [[Category: Anti-oncogene]]
 [[Category: Cell cycle]]

2k85

From Proteopedia

Revision as of 17:57, 25 December 2014

p190-A RhoGAP FF1 domain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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