1i3q
From Proteopedia
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|PDB= 1i3q |SIZE=350|CAPTION= <scene name='initialview01'>1i3q</scene>, resolution 3.1Å | |PDB= 1i3q |SIZE=350|CAPTION= <scene name='initialview01'>1i3q</scene>, resolution 3.1Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1en0|1EN0]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i3q OCA], [http://www.ebi.ac.uk/pdbsum/1i3q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i3q RCSB]</span> | ||
}} | }} | ||
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[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
[[Category: Kornberg, R D.]] | [[Category: Kornberg, R D.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: ZN]] | ||
[[Category: molecular machine]] | [[Category: molecular machine]] | ||
[[Category: mrna]] | [[Category: mrna]] | ||
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[[Category: zinc motif]] | [[Category: zinc motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:14:09 2008'' |
Revision as of 18:14, 30 March 2008
| |||||||
| , resolution 3.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Related: | 1EN0
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA POLYMERASE II CRYSTAL FORM I AT 3.1 A RESOLUTION
Overview
Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.
About this Structure
1I3Q is a Protein complex structure of sequences from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entry 1EN0. Full crystallographic information is available from OCA.
Reference
Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution., Cramer P, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1863-76. Epub 2001 Apr 19. PMID:11313498
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