2jaa

From Proteopedia

(Difference between revisions)

Revision as of 04:36, 25 December 2014

SEMET SUBSTITUTED SHIGELLA FLEXNERI IPAD

Structural highlights

2jaa is a 2 chain structure with sequence from Shigella flexneri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	2j0n, 2j0o
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[IPAD_SHIFL] Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via TTSS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Bacteria expressing type III secretion systems (T3SS) have been responsible for the deaths of millions worldwide, acting as key virulence elements in diseases ranging from plague to typhoid fever. The T3SS is composed of a basal body, which traverses both bacterial membranes, and an external needle through which effector proteins are secreted. We report multiple crystal structures of two proteins that sit at the tip of the needle and are essential for virulence: IpaD from Shigella flexneri and BipD from Burkholderia pseudomallei. The structures reveal that the N-terminal domains of the molecules are intramolecular chaperones that prevent premature oligomerization, as well as sharing structural homology with proteins involved in eukaryotic actin rearrangement. Crystal packing has allowed us to construct a model for the tip complex that is supported by mutations designed using the structure.

Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.,Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:17077085^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Menard R, Sansonetti P, Parsot C. The secretion of the Shigella flexneri Ipa invasins is activated by epithelial cells and controlled by IpaB and IpaD. EMBO J. 1994 Nov 15;13(22):5293-302. PMID:7957095
↑ Picking WL, Nishioka H, Hearn PD, Baxter MA, Harrington AT, Blocker A, Picking WD. IpaD of Shigella flexneri is independently required for regulation of Ipa protein secretion and efficient insertion of IpaB and IpaC into host membranes. Infect Immun. 2005 Mar;73(3):1432-40. PMID:15731041 doi:http://dx.doi.org/10.1128/IAI.73.3.1432-1440.2005
↑ Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM. Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD. J Biol Chem. 2007 Feb 9;282(6):4035-44. Epub 2006 Oct 31. PMID:17077085 doi:10.1074/jbc.M607945200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jaa"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2jaa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JAA FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j0n|2j0n]], [[2j0o|2j0o]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j0n|2j0n]], [[2j0o|2j0o]]</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jaa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jaa RCSB], [http://www.ebi.ac.uk/pdbsum/2jaa PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jaa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jaa RCSB], [http://www.ebi.ac.uk/pdbsum/2jaa PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/IPAD_SHIFL IPAD_SHIFL]] Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via TTSS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal.<ref>PMID:7957095</ref> <ref>PMID:15731041</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 30: / Line 32: @@
 </StructureSection>
 [[Category: Shigella flexneri]]
-[[Category: Birket, S.]]
+[[Category: Birket, S]]
-[[Category: Blocker, A J.]]
+[[Category: Blocker, A J]]
-[[Category: Deane, J E.]]
+[[Category: Deane, J E]]
-[[Category: Espina, M.]]
+[[Category: Espina, M]]
-[[Category: Field, T.]]
+[[Category: Field, T]]
-[[Category: Galyov, E E.]]
+[[Category: Galyov, E E]]
-[[Category: Johnson, S.]]
+[[Category: Johnson, S]]
-[[Category: Lea, S M.]]
+[[Category: Lea, S M]]
-[[Category: Olive, A.]]
+[[Category: Olive, A]]
-[[Category: Picking, W D.]]
+[[Category: Picking, W D]]
-[[Category: Picking, W L.]]
+[[Category: Picking, W L]]
-[[Category: Roversi, P.]]
+[[Category: Roversi, P]]
 [[Category: Cell invasion]]
 [[Category: Invasin]]
 [[Category: Ipad]]
 [[Category: Semet]]
-[[Category: Shigella flexneri]]
 [[Category: T3ss]]
 [[Category: Type iii secretion]]
 [[Category: Virulence]]

2jaa

From Proteopedia

Revision as of 04:36, 25 December 2014

SEMET SUBSTITUTED SHIGELLA FLEXNERI IPAD

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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