1ij6
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1ij6 |SIZE=350|CAPTION= <scene name='initialview01'>1ij6</scene>, resolution 3.1Å | |PDB= 1ij6 |SIZE=350|CAPTION= <scene name='initialview01'>1ij6</scene>, resolution 3.1Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ij5|1IJ5]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ij6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ij6 OCA], [http://www.ebi.ac.uk/pdbsum/1ij6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ij6 RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Sasaki, H.]] | [[Category: Sasaki, H.]] | ||
[[Category: Tanokura, M.]] | [[Category: Tanokura, M.]] | ||
| - | [[Category: CA]] | ||
[[Category: cbp40]] | [[Category: cbp40]] | ||
[[Category: fourty kda calcium binding protein]] | [[Category: fourty kda calcium binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:20:28 2008'' |
Revision as of 18:20, 30 March 2008
| |||||||
| , resolution 3.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 1IJ5
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CA2+-BOUND STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD
Overview
Acellular slime mold, Physarum polycephalum, has a unique wound-healing system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N terminus by a proteinase in plasmodia (CBP40delta), which does not aggregate in the Ca(2+)-bound form. Here we report the crystal structures of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both structures consist of three domains: coiled-coil, intervening, and EF-hand. The topology of the EF-hand domain is similar to that of calpain. The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands through a large hydrophobic interface, necessary for high Ca(2+) affinity. Conformational change upon Ca(2+) binding is small; however, the structure of CBP40delta provides novel insights into the mechanism of Ca(2+)-dependent oligomerization.
About this Structure
1IJ6 is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
Metal-free and Ca2+-bound structures of a multidomain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum., Iwasaki W, Sasaki H, Nakamura A, Kohama K, Tanokura M, Structure. 2003 Jan;11(1):75-85. PMID:12517342
Page seeded by OCA on Sun Mar 30 21:20:28 2008
