1ij5
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ij6|1IJ6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ij5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ij5 OCA], [http://www.ebi.ac.uk/pdbsum/1ij5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ij5 RCSB]</span> | ||
}} | }} | ||
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[[Category: fourty kda calcium binding protein]] | [[Category: fourty kda calcium binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:20:30 2008'' |
Revision as of 18:20, 30 March 2008
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| , resolution 3.0Å | |||||||
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| Related: | 1IJ6
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
METAL-FREE STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD
Overview
Acellular slime mold, Physarum polycephalum, has a unique wound-healing system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N terminus by a proteinase in plasmodia (CBP40delta), which does not aggregate in the Ca(2+)-bound form. Here we report the crystal structures of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both structures consist of three domains: coiled-coil, intervening, and EF-hand. The topology of the EF-hand domain is similar to that of calpain. The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands through a large hydrophobic interface, necessary for high Ca(2+) affinity. Conformational change upon Ca(2+) binding is small; however, the structure of CBP40delta provides novel insights into the mechanism of Ca(2+)-dependent oligomerization.
About this Structure
1IJ5 is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
Metal-free and Ca2+-bound structures of a multidomain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum., Iwasaki W, Sasaki H, Nakamura A, Kohama K, Tanokura M, Structure. 2003 Jan;11(1):75-85. PMID:12517342
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