1ij2
From Proteopedia
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|PDB= 1ij2 |SIZE=350|CAPTION= <scene name='initialview01'>1ij2</scene>, resolution 1.70Å | |PDB= 1ij2 |SIZE=350|CAPTION= <scene name='initialview01'>1ij2</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ij2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ij2 OCA], [http://www.ebi.ac.uk/pdbsum/1ij2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ij2 RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Kim, P S.]] | [[Category: Kim, P S.]] | ||
[[Category: Malashkevich, V N.]] | [[Category: Malashkevich, V N.]] | ||
| - | [[Category: ACE]] | ||
| - | [[Category: CD]] | ||
[[Category: gcn4 coiled coil]] | [[Category: gcn4 coiled coil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:20:30 2008'' |
Revision as of 18:20, 30 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
GCN4-pVTL Coiled-coil Trimer with Threonine at the a(16) position
Overview
Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.
About this Structure
1IJ2 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:11371197
Page seeded by OCA on Sun Mar 30 21:20:30 2008
