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| - | ==THE STRUCTURE OF THE RIBOSOME WITH ELONGATION FACTOR G TRAPPED IN THE POST-TRANSLOCATIONAL STATE (PART 2 OF 4).==
| + | #REDIRECT [[4v5f]] This PDB entry is obsolete and replaced by 4v5f |
| - | <StructureSection load='2wrj' size='340' side='right' caption='[[2wrj]], [[Resolution|resolution]] 3.60Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[2wrj]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The January 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WRJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WRJ FirstGlance]. <br>
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| - | </td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wri|2wri]], [[2wrk|2wrk]], [[2wrl|2wrl]], [[2wdi|2wdi]], [[2v47|2v47]], [[2wh4|2wh4]], [[2wh2|2wh2]], [[2wdl|2wdl]], [[2b9p|2b9p]], [[1yl3|1yl3]], [[2v49|2v49]], [[1giy|1giy]], [[2b66|2b66]], [[1v8q|1v8q]], [[2j03|2j03]], [[2j01|2j01]], [[2wdn|2wdn]], [[1gd8|1gd8]], [[1wki|1wki]], [[2b9n|2b9n]], [[2wdj|2wdj]], [[2wrr|2wrr]], [[2wrn|2wrn]], [[2wrq|2wrq]], [[2wro|2wro]]</td></tr>
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| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wrj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wrj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wrj RCSB], [http://www.ebi.ac.uk/pdbsum/2wrj PDBsum]</span></td></tr>
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| - | <table>
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| - | == Evolutionary Conservation ==
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| - | [[Image:Consurf_key_small.gif|200px|right]]
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| - | Check<jmol>
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| - | <jmolCheckbox>
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| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wr/2wrj_consurf.spt"</scriptWhenChecked>
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| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| - | <text>to colour the structure by Evolutionary Conservation</text>
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| - | </jmolCheckbox>
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| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| - | <div style="clear:both"></div>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Elongation factor G (EF-G) is a guanosine triphosphatase (GTPase) that plays a crucial role in the translocation of transfer RNAs (tRNAs) and messenger RNA (mRNA) during translation by the ribosome. We report a crystal structure refined to 3.6 angstrom resolution of the ribosome trapped with EF-G in the posttranslocational state using the antibiotic fusidic acid. Fusidic acid traps EF-G in a conformation intermediate between the guanosine triphosphate and guanosine diphosphate forms. The interaction of EF-G with ribosomal elements implicated in stimulating catalysis, such as the L10-L12 stalk and the L11 region, and of domain IV of EF-G with the tRNA at the peptidyl-tRNA binding site (P site) and with mRNA shed light on the role of these elements in EF-G function. The stabilization of the mobile stalks of the ribosome also results in a more complete description of its structure.
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| - | The structure of the ribosome with elongation factor G trapped in the posttranslocational state.,Gao YG, Selmer M, Dunham CM, Weixlbaumer A, Kelley AC, Ramakrishnan V Science. 2009 Oct 30;326(5953):694-9. PMID:19833919<ref>PMID:19833919</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | ==See Also==
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| - | *[[Escherichia coli LepA%2C the ribosomal back translocase|Escherichia coli LepA%2C the ribosomal back translocase]]
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| - | *[[Ribosome 3D structures|Ribosome 3D structures]]
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: 70S Ribosomes]]
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| - | [[Category: RCSB PDB Molecule of the Month]]
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| - | [[Category: Thermus thermophilus]]
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| - | [[Category: Dunham, C M.]]
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| - | [[Category: Gao, Y G.]]
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| - | [[Category: Kelley, A C.]]
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| - | [[Category: Ramakrishnan, V.]]
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| - | [[Category: Selmer, M.]]
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| - | [[Category: Weixlbaumer, A.]]
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| - | [[Category: Elongation factor g]]
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| - | [[Category: Protein synthesis]]
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| - | [[Category: Ribosome]]
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| - | [[Category: Translation]]
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| - | [[Category: Translocation]]
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