1j3p
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1j3p |SIZE=350|CAPTION= <scene name='initialview01'>1j3p</scene>, resolution 2.02Å | |PDB= 1j3p |SIZE=350|CAPTION= <scene name='initialview01'>1j3p</scene>, resolution 2.02Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=FE:FE (III) ION'>FE</scene> | + | |LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1j3q|1J3Q]], [[1j3r|1J3R]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3p OCA], [http://www.ebi.ac.uk/pdbsum/1j3p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j3p RCSB]</span> | ||
}} | }} | ||
| Line 29: | Line 32: | ||
[[Category: Shoun, H.]] | [[Category: Shoun, H.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
| - | [[Category: FE]] | ||
[[Category: archaea]] | [[Category: archaea]] | ||
[[Category: cupin superfamily]] | [[Category: cupin superfamily]] | ||
| Line 35: | Line 37: | ||
[[Category: phosphoglucose isomerase]] | [[Category: phosphoglucose isomerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:28:26 2008'' |
Revision as of 18:28, 30 March 2008
| |||||||
| , resolution 2.02Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Glucose-6-phosphate isomerase, with EC number 5.3.1.9 | ||||||
| Related: | 1J3Q, 1J3R
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Thermococcus litoralis phosphoglucose isomerase
Overview
The gene encoding phosphoglucose isomerase was cloned from Thermococcus litoralis, and functionally expressed in Escherichia coli. The purified enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized. The inhibition constants for four competitive inhibitors were determined. The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity was enhanced by the addition of Fe(2+), but inhibited by Zn(2+) and EDTA. Enzymes with mutations in conserved histidine and glutamate residues in their cupin motifs contained no metals, and showed large decreases in k(cat). The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.
About this Structure
1J3P is a Single protein structure of sequence from Thermococcus litoralis. Full crystallographic information is available from OCA.
Reference
Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis., Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T, FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:12560104
Page seeded by OCA on Sun Mar 30 21:28:26 2008
