1j4m
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1k43|1K43]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j4m OCA], [http://www.ebi.ac.uk/pdbsum/1j4m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j4m RCSB]</span> | ||
}} | }} | ||
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[[Category: beta-hairpin]] | [[Category: beta-hairpin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:28:45 2008'' |
Revision as of 18:28, 30 March 2008
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| Related: | 1K43
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Minimized average structure of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)
Overview
Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced.
About this Structure
1J4M is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides., Pastor MT, Lopez de la Paz M, Lacroix E, Serrano L, Perez-Paya E, Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):614-9. Epub 2002 Jan 8. PMID:11782528
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