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1jb7
From Proteopedia
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|PDB= 1jb7 |SIZE=350|CAPTION= <scene name='initialview01'>1jb7</scene>, resolution 1.86Å | |PDB= 1jb7 |SIZE=350|CAPTION= <scene name='initialview01'>1jb7</scene>, resolution 1.86Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= MAC-56A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]), MAC-41A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]) | |GENE= MAC-56A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]), MAC-41A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1otc|1otc]], [[156d|156d]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jb7 OCA], [http://www.ebi.ac.uk/pdbsum/1jb7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jb7 RCSB]</span> | ||
}} | }} | ||
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[[Category: Horvath, M P.]] | [[Category: Horvath, M P.]] | ||
[[Category: Schultz, S C.]] | [[Category: Schultz, S C.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: NA]] | ||
[[Category: dna hydration]] | [[Category: dna hydration]] | ||
[[Category: dna-protein interaction]] | [[Category: dna-protein interaction]] | ||
| Line 32: | Line 33: | ||
[[Category: telomere-binding protein]] | [[Category: telomere-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:31:05 2008'' |
Revision as of 18:31, 30 March 2008
| |||||||
| , resolution 1.86Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | MAC-56A (Sterkiella nova), MAC-41A (Sterkiella nova) | ||||||
| Related: | 1otc, 156d
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex
Overview
The Oxytricha nova telomere end binding protein (OnTEBP) recognizes, binds and protects the single-stranded 3'-terminal DNA extension found at the ends of macronuclear chromosomes. The structure of this complex shows that the single strand GGGGTTTTGGGG DNA binds in a deep cleft between the two protein subunits of OnTEBP, adopting a non-helical and irregular conformation. In extending the resolution limit of this structure to 1.86 A, we were surprised to find a G-quartet linked dimer of the GGGGTTTTGGGG DNA also packing within the crystal lattice and interacting with the telomere end binding protein. The G-quartet DNA exhibits the same structure and topology as previously observed in solution by NMR with diagonally crossing d(TTTT) loops at either end of the four-stranded helix. Additionally, the crystal structure reveals clearly visible Na(+), and specific patterns of bound water molecules in the four non-equivalent grooves. Although the G-quartet:protein contact surfaces are modest and might simply represent crystal packing interactions, it is interesting to speculate that the two types of telomeric DNA-protein interactions observed here might both be important in telomere biology.
About this Structure
1JB7 is a Protein complex structure of sequences from Sterkiella nova. Full crystallographic information is available from OCA.
Reference
DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:11428895
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