1jbt
From Proteopedia
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|PDB= 1jbt |SIZE=350|CAPTION= <scene name='initialview01'>1jbt</scene>, resolution 2.70Å | |PDB= 1jbt |SIZE=350|CAPTION= <scene name='initialview01'>1jbt</scene>, resolution 2.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1aqz|1AQZ]], [[430d|430D]], [[480d|480D]], [[483d|483D]], [[1jbr|1JBR]], [[1jbs|1JBS]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbt OCA], [http://www.ebi.ac.uk/pdbsum/1jbt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jbt RCSB]</span> | ||
}} | }} | ||
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[[Category: Glover, L.]] | [[Category: Glover, L.]] | ||
[[Category: Yang, X.]] | [[Category: Yang, X.]] | ||
| - | [[Category: K]] | ||
[[Category: highly specific ribonuclease]] | [[Category: highly specific ribonuclease]] | ||
[[Category: protein-rna recognition]] | [[Category: protein-rna recognition]] | ||
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[[Category: ribotoxin]] | [[Category: ribotoxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:31:23 2008'' |
Revision as of 18:31, 30 March 2008
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| , resolution 2.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Related: | 1AQZ, 430D, 480D, 483D, 1JBR, 1JBS
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF RIBOTOXIN RESTRICTOCIN COMPLEXED WITH A 29-MER SARCIN/RICIN DOMAIN RNA ANALOG
Overview
The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs that mimic the target sarcin/ricin loop (SRL) structure of the rat 28S rRNA. In these structures, restrictocin contacts the bulged-G motif and an unfolded form of the tetraloop of the SRL RNA. In one structure, toxin loops guide selection of the target site by contacting the base critical for recognition (G4319) and the surrounding S-shaped backbone. In another structure, base flipping of the tetraloop enables cleavage by placing the target nucleotide in the active site with the nucleophile nearly inline for attack on the scissile bond. These structures provide the first views of how a site-specific protein endonuclease recognizes and cleaves a folded RNA substrate.
About this Structure
1JBT is a Single protein structure of sequence from Aspergillus restrictus. Full crystallographic information is available from OCA.
Reference
Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping., Yang X, Gerczei T, Glover LT, Correll CC, Nat Struct Biol. 2001 Nov;8(11):968-73. PMID:11685244
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