1jiw
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1jiw |SIZE=350|CAPTION= <scene name='initialview01'>1jiw</scene>, resolution 1.74Å | |PDB= 1jiw |SIZE=350|CAPTION= <scene name='initialview01'>1jiw</scene>, resolution 1.74Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1smp|1SMP]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jiw OCA], [http://www.ebi.ac.uk/pdbsum/1jiw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jiw RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Gray, R D.]] | [[Category: Gray, R D.]] | ||
[[Category: Hege, T.]] | [[Category: Hege, T.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: ZN]] | ||
[[Category: pseudomonas aeruginosa alkaline protease inhibitor]] | [[Category: pseudomonas aeruginosa alkaline protease inhibitor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:34:23 2008'' |
Revision as of 18:34, 30 March 2008
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| , resolution 1.74Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1SMP
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the APR-APRin complex
Overview
Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.
About this Structure
1JIW is a Protein complex structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:11445573
Page seeded by OCA on Sun Mar 30 21:34:23 2008
