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2eqh

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Revision as of 02:16, 25 December 2014

Solution structure of growth-blocking peptide of the armyworm, Pseudaletia separata

Structural highlights

2eqh is a 1 chain structure with sequence from Mythimna separata. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2eqq, 2eqt
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[GBP_PSESE] Biogenic peptide that prevents the onset of metamorphosis from larva to pupa. Has repressive activity against juvenile hormone esterase. Induces cell proliferation at low concentrations and inhibits it at high concentrations. Mediates the spreading of plasmatocytes to foreign surfaces. May have a role in the regulation of dopamine concentrations.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Growth-blocking peptide (GBP) is a hormone-like peptide that suppresses the growth of the host armyworm. Although the 23-amino acid GBP (1-23 GBP) is expressed in nonparasitized armyworm plasma, the parasitization by wasp produces the 28-amino acid GBP (1-28 GBP) through an elongation of the C-terminal amino acid sequence. In this study, we characterized the GBP variants, which consist of various lengths of the C-terminal region, by comparing their biological activities and three-dimensional structures. The results of an injection study indicate that 1-28 GBP most strongly suppresses larval growth. NMR analysis shows that these peptides have basically the same tertiary structures and that the extension of the C-terminal region is disordered. However, the C-terminal region of 1-28 GBP undergoes a conformational transition from a random coiled state to an alpha-helical state in the presence of dodecylphosphocholine micelles. This suggests that binding of the C-terminal region would affect larval growth activity.

C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity.,Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K J Biol Chem. 2009 Oct 23;284(43):29625-34. Epub 2009 Aug 26. PMID:19710009^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hayakawa Y, Ohnishi A. Cell growth activity of growth-blocking peptide. Biochem Biophys Res Commun. 1998 Sep 18;250(2):194-9. PMID:9753606 doi:http://dx.doi.org/10.1006/bbrc.1998.8959
↑ Hayakawa Y, Ohnishi A, Mizoguchi A, Yamashika C. Distribution of growth-blocking peptide in the insect central nervous tissue. Cell Tissue Res. 2000 Jun;300(3):459-64. PMID:10928276
↑ Aizawa T, Hayakawa Y, Ohnishi A, Fujitani N, Clark KD, Strand MR, Miura K, Koganesawa N, Kumaki Y, Demura M, Nitta K, Kawano K. Structure and activity of the insect cytokine growth-blocking peptide. Essential regions for mitogenic and hemocyte-stimulating activities are separate. J Biol Chem. 2001 Aug 24;276(34):31813-8. Epub 2001 Jun 27. PMID:11429413 doi:http://dx.doi.org/10.1074/jbc.M105251200
↑ Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K. C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity. J Biol Chem. 2009 Oct 23;284(43):29625-34. Epub 2009 Aug 26. PMID:19710009 doi:10.1074/jbc.M109.011148

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2eqh"

@@ Line 6: / Line 6: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eqh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2eqh RCSB], [http://www.ebi.ac.uk/pdbsum/2eqh PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/GBP_PSESE GBP_PSESE]] Biogenic peptide that prevents the onset of metamorphosis from larva to pupa. Has repressive activity against juvenile hormone esterase. Induces cell proliferation at low concentrations and inhibits it at high concentrations. Mediates the spreading of plasmatocytes to foreign surfaces. May have a role in the regulation of dopamine concentrations.<ref>PMID:9753606</ref> <ref>PMID:10928276</ref> <ref>PMID:11429413</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 21: @@
 </StructureSection>
 [[Category: Mythimna separata]]
-[[Category: Aizawa, T.]]
+[[Category: Aizawa, T]]
-[[Category: Demura, M.]]
+[[Category: Demura, M]]
-[[Category: Kamiya, M.]]
+[[Category: Kamiya, M]]
-[[Category: Kawano, K.]]
+[[Category: Kawano, K]]
-[[Category: Kumaki, Y.]]
+[[Category: Kumaki, Y]]
-[[Category: Umetsu, Y.]]
+[[Category: Umetsu, Y]]
 [[Category: Cytokine]]
 [[Category: Growth-blocking peptide]]

2eqh

From Proteopedia

Revision as of 02:16, 25 December 2014

Solution structure of growth-blocking peptide of the armyworm, Pseudaletia separata

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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