1jr5
From Proteopedia
| Line 6: | Line 6: | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= asiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= asiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jr5 OCA], [http://www.ebi.ac.uk/pdbsum/1jr5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jr5 RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1JR5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1JR5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR5 OCA]. |
==Reference== | ==Reference== | ||
Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions., Urbauer JL, Simeonov MF, Urbauer RJ, Adelman K, Gilmore JM, Brody EN, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1831-5. Epub 2002 Feb 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11830637 11830637] | Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions., Urbauer JL, Simeonov MF, Urbauer RJ, Adelman K, Gilmore JM, Brody EN, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1831-5. Epub 2002 Feb 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11830637 11830637] | ||
| - | [[Category: | + | [[Category: Enterobacteria phage t4]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Adelman, K.]] | [[Category: Adelman, K.]] | ||
| Line 32: | Line 35: | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:37:50 2008'' |
Revision as of 18:37, 30 March 2008
| |||||||
| Gene: | asiA (Enterobacteria phage T4) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of the Anti-Sigma Factor AsiA Homodimer
Overview
Anti-sigma factors regulate prokaryotic gene expression through interactions with specific sigma factors. The bacteriophage T4 anti-sigma factor AsiA is a molecular switch that both inhibits transcription from bacterial promoters and phage early promoters and promotes transcription at phage middle promoters through its interaction with the primary sigma factor of Escherichia coli, sigma(70). AsiA is an all-helical, symmetric dimer in solution. The solution structure of the AsiA dimer reveals a novel helical fold for the protomer. Furthermore, the AsiA protomer, surprisingly, contains a helix-turn-helix DNA binding motif, predicting a potential new role for AsiA. The AsiA dimer interface includes a substantial hydrophobic component, and results of hydrogen/deuterium exchange studies suggest that the dimer interface is the most stable region of the AsiA dimer. In addition, the residues that form the dimer interface are those that are involved in binding to sigma(70). The results promote a model whereby the AsiA dimer maintains the active hydrophobic surfaces and delivers them to sigma(70), where an AsiA protomer is displaced from the dimer via the interaction of sigma(70) with the same residues in AsiA that constitute the dimer interface.
About this Structure
1JR5 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions., Urbauer JL, Simeonov MF, Urbauer RJ, Adelman K, Gilmore JM, Brody EN, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1831-5. Epub 2002 Feb 5. PMID:11830637
Page seeded by OCA on Sun Mar 30 21:37:50 2008
