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2xsy

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REDIRECT 4v5m This PDB entry is obsolete and replaced by 4v5m

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-==tRNA tranlocation on the 70S ribosome: the pre-translocational translocation intermediate TI(PRE)==
+#REDIRECT [[4v5m]] This PDB entry is obsolete and replaced by 4v5m
-<StructureSection load='2xsy' size='340' side='right' caption='[[2xsy]], [[Resolution|resolution]] 7.80&Aring;' scene=''>
-== Structural highlights ==
-<table><tr><td colspan='2'>[[2xsy]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XSY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUA:FUSIDIC+ACID'>FUA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xtg|2xtg]], [[1ibk|1ibk]], [[1ibl|1ibl]], [[2uuc|2uuc]], [[2wdk|2wdk]], [[2j02|2j02]], [[1j5e|1j5e]], [[2wrq|2wrq]], [[1hnx|1hnx]], [[2j00|2j00]], [[2b9o|2b9o]], [[2x9t|2x9t]], [[2uub|2uub]], [[2wh3|2wh3]], [[1vov|1vov]], [[1emi|1emi]], [[2xqd|2xqd]], [[2v46|2v46]], [[1pnx|1pnx]], [[2efg|2efg]], [[2vqf|2vqf]], [[2wh1|2wh1]], [[1n32|1n32]], [[1elo|1elo]], [[2wrn|2wrn]], [[2jl5|2jl5]], [[2f4v|2f4v]], [[1twt|1twt]], [[1qzc|1qzc]], [[2uxd|2uxd]], [[1fka|1fka]], [[1fjg|1fjg]], [[1n36|1n36]], [[2uxc|2uxc]], [[1hnw|1hnw]], [[1l1u|1l1u]], [[1hr0|1hr0]], [[2v48|2v48]], [[2b64|2b64]], [[1xmq|1xmq]], [[1dar|1dar]], [[2b9m|2b9m]], [[1pn8|1pn8]], [[2wdg|2wdg]], [[1xnr|1xnr]], [[1dv4|1dv4]], [[1hnz|1hnz]], [[1rss|1rss]], [[2wdh|2wdh]], [[1jgq|1jgq]], [[1i94|1i94]], [[1i96|1i96]], [[2uu9|2uu9]], [[1pn7|1pn7]], [[1eg0|1eg0]], [[1ibm|1ibm]], [[2uxb|2uxb]], [[2vqe|2vqe]], [[1g1x|1g1x]], [[2uua|2uua]], [[1i95|1i95]], [[1xnq|1xnq]], [[2xfz|2xfz]], [[1qd7|1qd7]], [[1gix|1gix]], [[1efg|1efg]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[2xg1|2xg1]], [[2x9r|2x9r]], [[1n34|1n34]], [[1n33|1n33]], [[1pns|1pns]], [[2jl7|2jl7]], [[1yl4|1yl4]], [[2wdm|2wdm]], [[1xmo|1xmo]], [[1i97|1i97]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xsy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xsy RCSB], [http://www.ebi.ac.uk/pdbsum/2xsy PDBsum]</span></td></tr>
-</table>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-The elongation cycle of protein synthesis involves the delivery of aminoacyl-transfer RNAs to the aminoacyl-tRNA-binding site (A site) of the ribosome, followed by peptide-bond formation and translocation of the tRNAs through the ribosome to reopen the A site. The translocation reaction is catalysed by elongation factor G (EF-G) in a GTP-dependent manner. Despite the availability of structures of various EF-G-ribosome complexes, the precise mechanism by which tRNAs move through the ribosome still remains unclear. Here we use multiparticle cryoelectron microscopy analysis to resolve two previously unseen subpopulations within Thermus thermophilus EF-G-ribosome complexes at subnanometre resolution, one of them with a partly translocated tRNA. Comparison of these substates reveals that translocation of tRNA on the 30S subunit parallels the swivelling of the 30S head and is coupled to unratcheting of the 30S body. Because the tRNA maintains contact with the peptidyl-tRNA-binding site (P site) on the 30S head and simultaneously establishes interaction with the exit site (E site) on the 30S platform, a novel intra-subunit 'pe/E' hybrid state is formed. This state is stabilized by domain IV of EF-G, which interacts with the swivelled 30S-head conformation. These findings provide direct structural and mechanistic insight into the 'missing link' in terms of tRNA intermediates involved in the universally conserved translocation process.
-Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites.,Ratje AH, Loerke J, Mikolajka A, Brunner M, Hildebrand PW, Starosta AL, Donhofer A, Connell SR, Fucini P, Mielke T, Whitford PC, Onuchic JN, Yu Y, Sanbonmatsu KY, Hartmann RK, Penczek PA, Wilson DN, Spahn CM Nature. 2010 Dec 2;468(7324):713-6. PMID:21124459<ref>PMID:21124459</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-==See Also==
-*[[Elongation factor|Elongation factor]]
-== References ==
-<references/>
-__TOC__
-</StructureSection>
-[[Category: Thermus thermophilus]]
-[[Category: Bruenner, M.]]
-[[Category: Connell, S R.]]
-[[Category: Doenhoefer, A.]]
-[[Category: Fucini, P.]]
-[[Category: Hartmann, R K.]]
-[[Category: Hildebrand, P W.]]
-[[Category: Loerke, J.]]
-[[Category: Mielke, T.]]
-[[Category: Mikolajka, A.]]
-[[Category: Onuchic, J N.]]
-[[Category: Penczek, P A.]]
-[[Category: Ratje, A H.]]
-[[Category: Sanbonmatsu, K Y.]]
-[[Category: Spahn, C M.T.]]
-[[Category: Starosta, A L.]]
-[[Category: Whitford, P C.]]
-[[Category: Wilson, D N.]]
-[[Category: Yu, Y.]]
-[[Category: Elongation cycle]]
-[[Category: Ribosome]]
-[[Category: Translation]]

2xsy

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