4cza
From Proteopedia
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| - | ''' | + | ==Structure of the sodium proton antiporter PaNhaP from Pyrococcus abyssii with bound thallium ion.== |
| + | <StructureSection load='4cza' size='340' side='right' caption='[[4cza]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4cza]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CZA FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=TAM:TRIS(HYDROXYETHYL)AMINOMETHANE'>TAM</scene>, <scene name='pdbligand=TL:THALLIUM+(I)+ION'>TL</scene>, <scene name='pdbligand=UND:UNDECANE'>UND</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cz8|4cz8]], [[4cz9|4cz9]], [[4czb|4czb]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cza OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cza RCSB], [http://www.ebi.ac.uk/pdbsum/4cza PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sodium/proton antiporters maintain intracellular pH and sodium levels. Detailed structures of antiporters with bound substrate ions are essential for understanding how they work. We have resolved the substrate ion in the dimeric, electroneutral sodium/proton antiporter PaNhaP from Pyrococcus abyssi at 3.2 A, and have determined its structure in two different conformations at pH 8 and pH 4. The ion is coordinated by three acidic sidechains, a water molecule, a serine and a main-chain carbonyl in the unwound stretch of trans-membrane helix 5 at the deepest point of a negatively charged cytoplasmic funnel. A second narrow polar channel may facilitate proton uptake from the cytoplasm. Transport activity of PaNhaP is cooperative at pH 6 but not at pH 5. Cooperativity is due to pH-dependent allosteric coupling of protomers through two histidines at the dimer interface. Combined with comprehensive transport studies, the structures of PaNhaP offer unique new insights into the transport mechanism of sodium/proton antiporters. | ||
| - | + | Structure and substrate ion binding in the sodium/proton antiporter PaNhaP.,Wohlert D, Kuhlbrandt W, Yildiz O Elife. 2014 Nov 26;3. doi: 10.7554/eLife.03579. PMID:25426802<ref>PMID:25426802</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Kuhlbrandt, W]] | ||
| + | [[Category: Woehlert, D]] | ||
| + | [[Category: Yildiz, O]] | ||
| + | [[Category: Cpa]] | ||
| + | [[Category: Exchanger]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Transporter]] | ||
Revision as of 12:42, 17 December 2014
Structure of the sodium proton antiporter PaNhaP from Pyrococcus abyssii with bound thallium ion.
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