1k3y
From Proteopedia
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|PDB= 1k3y |SIZE=350|CAPTION= <scene name='initialview01'>1k3y</scene>, resolution 1.30Å | |PDB= 1k3y |SIZE=350|CAPTION= <scene name='initialview01'>1k3y</scene>, resolution 1.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1k3l|1K3L]], [[1k3o|1K3O]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3y OCA], [http://www.ebi.ac.uk/pdbsum/1k3y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k3y RCSB]</span> | ||
}} | }} | ||
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[[Category: Stenkamp, R E.]] | [[Category: Stenkamp, R E.]] | ||
[[Category: Trong, I Le.]] | [[Category: Trong, I Le.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: GTX]] | ||
[[Category: glutathione s-transferase]] | [[Category: glutathione s-transferase]] | ||
[[Category: s-hexyl glutatione]] | [[Category: s-hexyl glutatione]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:43:05 2008'' |
Revision as of 18:43, 30 March 2008
| |||||||
| , resolution 1.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Glutathione transferase, with EC number 2.5.1.18 | ||||||
| Related: | 1K3L, 1K3O
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Analysis of human Glutathione S-transferase with S-hexyl glutatione and glycerol at 1.3 Angstrom
Overview
Cytosolic glutathione S-transferases (GSTs) play a critical role in xenobiotic binding and metabolism, as well as in modulation of oxidative stress. Here, the high-resolution X-ray crystal structures of homodimeric human GSTA1-1 in the apo form and in complex with S-hexyl glutathione (two data sets) are reported at 1.8, 1.5, and 1.3A respectively. At this level of resolution, distinct conformations of the alkyl chain of S-hexyl glutathione are observed, reflecting the nonspecific nature of the hydrophobic substrate binding site (H-site). Also, an extensive network of ordered water, including 75 discrete solvent molecules, traverses the open subunit-subunit interface and connects the glutathione binding sites in each subunit. In the highest-resolution structure, three glycerol moieties lie within this network and directly connect the amino termini of the glutathione molecules. A search for ligand binding sites with the docking program Molecular Operating Environment identified the ordered water network binding site, lined mainly with hydrophobic residues, suggesting an extended ligand binding surface for nonsubstrate ligands, the so-called ligandin site. Finally, detailed comparison of the structures reported here with previously published X-ray structures reveal a possible reaction coordinate for ligand-dependent conformational changes in the active site and the C-terminus.
About this Structure
1K3Y is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
1.3-A resolution structure of human glutathione S-transferase with S-hexyl glutathione bound reveals possible extended ligandin binding site., Le Trong I, Stenkamp RE, Ibarra C, Atkins WM, Adman ET, Proteins. 2002 Sep 1;48(4):618-27. PMID:12211029
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