1kio
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1kgm|1KGM]], [[1pmc|1PMC]], [[1kj0|1KJ0]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kio OCA], [http://www.ebi.ac.uk/pdbsum/1kio PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kio RCSB]</span> | ||
}} | }} | ||
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[[Category: specificity]] | [[Category: specificity]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:49:08 2008'' |
Revision as of 18:49, 30 March 2008
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| Related: | 1KGM, 1PMC, 1KJ0
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]
Overview
The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit different specificities towards target proteinases, where SGCI is a good chymotrypsin inhibitor, its mutant is a potent trypsin inhibitor, and SGTI inhibits both proteinases weakly. Interestingly, SGTI is a much better inhibitor of insect proteinases than of the mammalian ones used in common assays. All three molecules have a similar fold composed from three antiparallel beta-pleated sheets with three disulfide bridges. The proteinase binding loop has a somewhat distinct geometry in all three peptides. Moreover, the stabilization of the structure is different in SGCI and SGTI. Proton-deuterium exchange experiments are indicative of a highly rigid core in SGTI but not in SGCI. We suggest that the observed structural properties play a significant role in the specificity of these inhibitors.
About this Structure
1KIO is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria., Gaspari Z, Patthy A, Graf L, Perczel A, Eur J Biochem. 2002 Jan;269(2):527-37. PMID:11856311
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