1kpe
From Proteopedia
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|PDB= 1kpe |SIZE=350|CAPTION= <scene name='initialview01'>1kpe</scene>, resolution 1.8Å | |PDB= 1kpe |SIZE=350|CAPTION= <scene name='initialview01'>1kpe</scene>, resolution 1.8Å | ||
|SITE= <scene name='pdbsite=AVA:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVA</scene>, <scene name='pdbsite=AVB:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVB</scene>, <scene name='pdbsite=HNA:HIS+Triad+For+Which+This+Family+Was+Named'>HNA</scene> and <scene name='pdbsite=HNB:HIS+Triad+For+Which+This+Family+Was+Named'>HNB</scene> | |SITE= <scene name='pdbsite=AVA:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVA</scene>, <scene name='pdbsite=AVB:Active+Site+HIS+Responsible+For+Forming+The+Transient+Nu+...'>AVB</scene>, <scene name='pdbsite=HNA:HIS+Triad+For+Which+This+Family+Was+Named'>HNA</scene> and <scene name='pdbsite=HNB:HIS+Triad+For+Which+This+Family+Was+Named'>HNB</scene> | ||
| - | |LIGAND= <scene name='pdbligand=ADW:ADENOSINE-5 | + | |LIGAND= <scene name='pdbligand=ADW:ADENOSINE-5'-DITUNGSTATE'>ADW</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= HPKCI-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= HPKCI-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
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[[Category: protein kinase inhibitor]] | [[Category: protein kinase inhibitor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:31:11 2008'' |
Revision as of 10:31, 23 March 2008
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| , resolution 1.8Å | |||||||
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| Sites: | , , and | ||||||
| Ligands: | |||||||
| Gene: | HPKCI-1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PKCI-TRANSITION STATE ANALOG
Overview
The histidine triad (HIT) protein family is among the most ubiquitous and highly conserved in nature, but a biological activity has not yet been identified for any member of the HIT family. Fragile histidine triad protein (FHIT) and protein kinase C interacting protein (PKCI) were used in a structure-based approach to elucidate characteristics of in vivo ligands and reactions. Crystallographic structures of apo, substrate analog, pentacovalent transition-state analog, and product states of both enzymes reveal a catalytic mechanism and define substrate characteristics required for catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both. The approach described here may be useful in identifying structure-function relations between protein families identified through genomics.
About this Structure
1KPE is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure-based analysis of catalysis and substrate definition in the HIT protein family., Lima CD, Klein MG, Hendrickson WA, Science. 1997 Oct 10;278(5336):286-90. PMID:9323207
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