1kqr
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1kqr |SIZE=350|CAPTION= <scene name='initialview01'>1kqr</scene>, resolution 1.4Å | |PDB= 1kqr |SIZE=350|CAPTION= <scene name='initialview01'>1kqr</scene>, resolution 1.4Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MNA:2-O-METHYL-5-N-ACETYL-ALPHA-D-+NEURAMINIC+ACID'>MNA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= segment 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10969 Rhesus rotavirus]) | |GENE= segment 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10969 Rhesus rotavirus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1kri|1KRI]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kqr OCA], [http://www.ebi.ac.uk/pdbsum/1kqr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kqr RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Sun, Z Y.J.]] | [[Category: Sun, Z Y.J.]] | ||
[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: MNA]] | ||
| - | [[Category: SO4]] | ||
[[Category: cell attachment]] | [[Category: cell attachment]] | ||
[[Category: galectin fold]] | [[Category: galectin fold]] | ||
| Line 41: | Line 41: | ||
[[Category: vp8*]] | [[Category: vp8*]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:52:14 2008'' |
Revision as of 18:52, 30 March 2008
| |||||||
| , resolution 1.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | segment 4 (Rhesus rotavirus) | ||||||
| Related: | 1KRI
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Rhesus Rotavirus VP4 Sialic Acid Binding Domain in Complex with 2-O-methyl-alpha-D-N-acetyl neuraminic acid
Overview
Cell attachment and membrane penetration are functions of the rotavirus outer capsid spike protein, VP4. An activating tryptic cleavage of VP4 produces the N-terminal fragment, VP8*, which is the viral hemagglutinin and an important target of neutralizing antibodies. We have determined, by X-ray crystallography, the atomic structure of the VP8* core bound to sialic acid and, by NMR spectroscopy, the structure of the unliganded VP8* core. The domain has the beta-sandwich fold of the galectins, a family of sugar binding proteins. The surface corresponding to the galectin carbohydrate binding site is blocked, and rotavirus VP8* instead binds sialic acid in a shallow groove between its two beta-sheets. There appears to be a small induced fit on binding. The residues that contact sialic acid are conserved in sialic acid-dependent rotavirus strains. Neutralization escape mutations are widely distributed over the VP8* surface and cluster in four epitopes. From the fit of the VP8* core into the virion spikes, we propose that VP4 arose from the insertion of a host carbohydrate binding domain into a viral membrane interaction protein.
About this Structure
1KQR is a Single protein structure of sequence from Rhesus rotavirus. Full crystallographic information is available from OCA.
Reference
The rhesus rotavirus VP4 sialic acid binding domain has a galectin fold with a novel carbohydrate binding site., Dormitzer PR, Sun ZY, Wagner G, Harrison SC, EMBO J. 2002 Mar 1;21(5):885-97. PMID:11867517
Page seeded by OCA on Sun Mar 30 21:52:14 2008
